Detection of single biomolecule fluorescence excited through energy transfer:: Application to light-harvesting complexes

被引：21

作者：

Woermke, S.

Mackowski, S.

Brotosudarmo, T. H. P.

Braeuchle, Ch.

Garcia, A.

Braun, P.

Scheer, H.

Hofmann, E.

机构：

[1] Univ Munich, Dept Chem & Biochem, D-81377 Munich, Germany

[2] Univ Munich, Ctr Nanosci, D-81377 Munich, Germany

[3] Univ Munich, Dept Biol, D-80638 Munich, Germany

[4] Ruhr Univ Bochum, Dept Biol, D-44780 Bochum, Germany

来源：

APPLIED PHYSICS LETTERS | 2007年 / 90卷 / 19期

关键词：

D O I：

10.1063/1.2737429

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The authors show that energy transfer is a feasible mechanism for exciting fluorescence of single light-harvesting complexes with different characters of the fluorescing state. This approach is applied for excitons consisting of 18 strongly coupled bacteriochlorophylls in light-harvesting complex 2 as well as for peridinin-chlorophyll-protein monomers containing either two chlorophyll a molecules or a pair of chlorophyll a and chlorophyll b characterized with the emission energy difference of 400 cm(-1). Using this method the authors are able to observe fluorescence spectral dynamics on the scale comparable or larger than the emission linewidth of a single chromophore. (C) 2007 American Institute of Physics.

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页数：3

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