Simultaneous non-instrumental detection of aflatoxin B1 and ochratoxin A using a clean-up tandem immunoassay column

被引：65

作者：

Goryacheva, Irina Yu. ^{[1
,2
]}

De Saeger, Sarah ^{[1
]}

Delmulle, Barbara ^{[1
]}

Lobeau, Marieke ^{[1
]}

Eremin, Sergei A. ^{[3
]}

Barna-Vetro, Ildiko ^{[4
]}

Van Peteghem, Carlos ^{[1
]}

机构：

[1] Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, B-9000 Ghent, Belgium

[2] Saratov NG Chernyshevskii State Univ, Fac Chem, Saratov 410012, Russia

[3] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119899, Russia

[4] Agr Biotechnol Ctr, Diagnost Lab, H-2101 Godollo, Hungary

来源：

ANALYTICA CHIMICA ACTA | 2007年 / 590卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

multiple analysis; immunoassay; rapid methods; Mycotoxins; ochratoxin a; aflatoxin B 1; spices;

D O I：

10.1016/j.aca.2007.02.075

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A set-up and simple method based on the clean-up tandem immunoassay approach was developed for the visual detection of two analytes. The method was based on a 1 mL column with one clean-up layer and two detection immunolayers. As detection immunolayers CNBr-activated Sepharose 413 with coupled secondary rabbit anti-mouse antibodies was used. Different specific antibodies were coupled to each detection immunolayer. The analysis was realised in a competitive ELISA format with visual detection of the developed colour for each detection immunolayer and took 20 min for six sample extracts. The described method was applied to the simultaneous detection of aflatoxin B 1 and ochratoxin A in spices with cut-off levels at 5 and 10 mu g kg(-1), respectively. Results were confirmed by LC-MS/MS with immunoaffinity column clean-up. (c) 2007 Published by Elsevier B.V.

引用

页码：118 / 124

页数：7

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