A general method for gene isolation in tagging approaches: amplification of insertion mutagenised sites (AIMS)

被引：50

作者：

Frey, M

Stettner, C

Gierl, A

机构：

[1] Tech Univ Munich, Genet Inst, D-85747 Garching, Germany

[2] Tech Univ Munich, Lehrstuhl Genet, D-85747 Garching, Germany

来源：

PLANT JOURNAL | 1998年 / 13卷 / 05期

关键词：

D O I：

10.1046/j.1365-313X.1998.00091.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T-DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The technique has been used successfully for the isolation of the maize Bx1 gene tagged by a Mutator element.

引用

页码：717 / 721

页数：5

共 13 条

[1] CHOMET P, 1994, MAIZE HDB, P23
[2] T-DNA INSERTION MUTAGENESIS IN ARABIDOPSIS - MUTATIONAL SPECTRUM
FELDMANN, KA
[J]. PLANT JOURNAL, 1991, 1 (01) : 71 - 82
[3] EXPRESSION OF A CYTOCHROME-P450 GENE FAMILY IN MAIZE
FREY, M
KLIEM, R
SAEDLER, H
GIERL, A
[J]. MOLECULAR & GENERAL GENETICS, 1995, 246 (01): : 100 - 109
[4] Analysis of a chemical plant defense mechanism in grasses
Frey, M
Chomet, P
Glawischnig, E
Stettner, C
Grun, S
Winklmair, A
Eisenreich, W
Bacher, A
Meeley, RB
Briggs, SP
Simcox, K
Gierl, A
[J]. SCIENCE, 1997, 277 (5326) : 696 - 699
[5] PLANT-TRANSPOSABLE ELEMENTS AND GENE TAGGING
GIERL, A
SAEDLER, H
[J]. PLANT MOLECULAR BIOLOGY, 1992, 19 (01) : 39 - 49
[6] HAMILTON R. H., 1964, WEEDS, V12, P27, DOI 10.2307/4040633
[7] KONLECZNY A, 1993, PLANT J, V4, P403
[8] KUNZE R, 1997, ADV BOT RES, V27, P321
[9] Sambrook J., 2002, MOL CLONING LAB MANU
[10] LOCATION OF THE BENZOXAZINLESS (BX) LOCUS IN MAIZE BY MONOSOMIC AND B-A TRANSLOCATIONAL ANALYSES
SIMCOX, KD
WEBER, DF
[J]. CROP SCIENCE, 1985, 25 (05) : 827 - 830

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