Decreased neutrophil bactericidal activity during phagocytosis of a slime-producing Staphylococcus aureus strain

被引：20

作者：

Barrio, B ^{[1
]}

Vangroenweghe, F ^{[1
]}

Dosogne, H ^{[1
]}

Burvenich, C ^{[1
]}

机构：

[1] State Univ Ghent, Fac Vet Med, Dept Physiol Biochem & Biometr, B-9820 Merelbeke, Belgium

来源：

VETERINARY RESEARCH | 2000年 / 31卷 / 06期

关键词：

bovine blood neutrophil; Staphylococcus aureus; slime; bacteriological assay;

D O I：

10.1051/vetres:2000143

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Phagocytosis and intracellular killing by bovine polymorphonuclear leukocytes (PMN) are important host defence mechanisms against mastitis caused by Staphylococcus aureus. We compared the phagocytosis and overall killing of a non slime-producing (NSP) S. aureus and its slime-producing (SP) variant by blood PMN, using an in vitro bacteriological assay. Seven clinically healthy Holstein-Friesian dairy cows in mid-lactation stage were used for this purpose. The percentages of overall killing for the NSP and SP variant were 34 +/- 3% and 21 +/- 4% (P < 0.05) and the corresponding percentages of phagocytosis were 40 +/- 4% and 31 +/- 4%, respectively. A significant positive correlation (r = 0.79; P < 0.001) was found between phagocytosis and overall killing. These results suggest that the presence of slime was responsible for a decreased phagocytic ingestion and overall killing.

引用

页码：603 / 609

页数：7

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