Identification of components of traditional Chinese medicine preparation "Jing-Zhi-Guan-Xin" troche by medium pressure LC and HPLC-DAD-MS

被引：11

作者：

Li, Yunfei ^{[1
]}

Liu, Li ^{[1
]}

Cheng, Yiyu ^{[1
]}

机构：

[1] Zhejiang Univ, Pharmaceut Informat Inst, Coll Pharmaceut Sci, Hangzhou 310027, Peoples R China

来源：

CHROMATOGRAPHIA | 2007年 / 65卷 / 11-12期

关键词：

column liquid chromatography; HPLC-DAD-MS; medium pressure liquid chromatography; Jing-Zhi-Guan-Xin" troche preparation;

D O I：

10.1365/s10337-007-0207-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comprehensive identification of the phytochemical components is one of the key points in the study on traditional Chinese medicine (TCM). In the present study, an approach combining separation and identification of the complex chemical composition in a TCM preparation named "Jing-Zhi-Guan-Xin" (JZGX) troche was developed. Medium pressure liquid chromatography (MPLC) was used to separate JZGX troche into several fractions according to their different polarity. Then, HPLC-DAD-MS was performed to acquire MS and UV spectra of the components within each of the separated fractions. Finally, 64 components were detected, among which, 22 components were identified by comparing their obtained retention times, molecular weights and UV spectra with the available standards and reference data. The results indicate that this approach is beneficial to explore chemical composition of traditional Chinese medicine preparation.

引用

页码：749 / 755

页数：7

共 20 条

[1] Determination of saikosaponin derivatives in Radix bupleuri and in pharmaceuticals of the Chinese multiherb remedy Xiaochaihu-tang using liquid chromatographic tandem mass spectrometry [J].

Bao, YW ;

Li, CA ;

Shen, HW ;

Nan, FJ .

ANALYTICAL CHEMISTRY, 2004, 76 (14) :4208-4216

[2] Determination of daphnetin in Daphne tangutica and its medicinal preparation by liquid chromatography [J].

Chen, J ;

Liu, X ;

Shi, YP .

ANALYTICA CHIMICA ACTA, 2004, 523 (01) :29-33

[3] Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia [J].

Chen, XG ;

Kong, L ;

Su, XY ;

Pan, CS ;

Ye, ML ;

Zou, HF .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1089 (1-2) :87-100

[4] Separation and identification of compounds in Rhizoma chuanxiong by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry [J].

Chen, XG ;

Kong, L ;

Su, XY ;

Fu, HJ ;

Ni, JY ;

Zhao, RH ;

Zou, HF .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1040 (02) :169-178

[5] Fast determination of curcumol, curdione and germacrone in three species of Curcuma rhizomes by microwave-assisted extraction followed by headspace solid-phase microextraction and gas chromatography-mass spectrometry [J].

Deng, Chunhui ;

Ji, Jie ;

Li, Ning ;

Yu, Yingjia ;

Duan, Gengli ;

Zhang, Xiangmin .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1117 (02) :115-120

[6] Isolation and characterization of impurities in docetaxel [J].

Dev, RV ;

Babu, JM ;

Vyas, K ;

Ram, PS ;

Ramachandra, P ;

Sekhar, NM ;

Reddy, DNM ;

Rao, NS .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2006, 40 (03) :614-622

[7] Analysis of 'SHUANGDAN' granules by high-performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry [J].

He, Qing ;

Hu, Xing-Jiang ;

Cheng, Yi-Yu .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2006, 41 (02) :485-492

[8] Preparative isolation and purification of six diterpenoids from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography [J].

Li, HB ;

Chen, F .

JOURNAL OF CHROMATOGRAPHY A, 2001, 925 (1-2) :109-114

[9] Determination of baicalin, chlorogenic acid and forsythin in Chinese medicinal preparation by capillary electrophoresis (vol 55, pg 453, 2002) [J].

Li, XJ ;

Zhang, YP ;

Yuan, ZB .

CHROMATOGRAPHIA, 2002, 55 (11-12) :768-768

[10] Study on the therapeutic material basis of Traditional Chinese Medicinal Preparation Suanzaoren decoction [J].

Li, Yu-Juan ;

Bi, Kai-Shun .

CHEMICAL & PHARMACEUTICAL BULLETIN, 2006, 54 (06) :847-851

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