Merkel cell carcinoma subgroups by Merkel cell polyomavirus DNA relative abundance and oncogene expression

被引:112
作者
Bhatia, Kishor [1 ]
Goedert, James J. [1 ]
Modali, Rama [2 ]
Preiss, Liliana [3 ]
Ayers, Leona W. [4 ]
机构
[1] NCI, Infect & Immunoepidemiol Branch, Div Canc Epidemiol & Genet, Rockville, MD USA
[2] Bioserve Biotechnol Ltd, Laurel, MD USA
[3] RTI Int, Rockville, MD USA
[4] Ohio State Univ, Coll Med, Dept Pathol, Columbus, OH 43210 USA
关键词
Merkel cell carcinoma; MCPyV; oncogenesis; skin cancer; POTENTIAL DIAGNOSTIC PITFALL; ENDOTHELIAL-CELLS; HUMAN CANCER; T-ANTIGEN; SARCOMA; SV40; INFECTION; GENE; SKIN; TDT;
D O I
10.1002/ijc.24676
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Merkel cell polyomavirus (MCPyV) was recently discovered in Merkel cell carcinoma (MCC), a clinically and pathologically heterogeneous malignancy of dermal neuroendocrine cells. To investigate this heterogeneity, we developed a tissue microarray (TMA) to characterize immunohistochemical staining of candidate tumor cell proteins and a quantitative PCR assay to detect MCPyV and measure viral loads. MCPyV was detected in 19 of 23 (74%) primary MCC tumors, but 8 of these had less than 1 viral copy per 300 cells. Viral abundance of 0.06-1.2 viral copies/cell was directly related to presence of retinoblastoma gene product (pRb) and terminal deoxyribonucleotidyl transferase (TdT) by immunohistochemical staining (p < 0.003). Higher viral abundance tumors tended to be associated with less p53 expression, younger age at diagnosis and longer survival (p < 0.08). These data suggest that MCC may arise through different oncogenic pathways, including ones independent of pRb and MCPyV.
引用
收藏
页码:2240 / 2246
页数:7
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