Heat shock response by the hyperthermophilic archaeon Pyrococcus furiosus

被引:89
作者
Shockley, KR [1 ]
Ward, DE [1 ]
Chhabra, SR [1 ]
Conners, SB [1 ]
Montero, CI [1 ]
Kelly, RM [1 ]
机构
[1] N Carolina State Univ, Dept Chem Engn, Raleigh, NC 27695 USA
关键词
D O I
10.1128/AEM.69.4.2365-2371.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Collective transcriptional analysis of heat shock response in the hyperthermophilic archaeon Pyrococcus furiosus was examined by using a targeted cDNA microarray in conjunction with Northern analyses. Differential gene expression suggests that P.furiosus relies on a cooperative strategy of rescue (thermosome [Hsp60], small heat shock protein [Hsp20], and two VAT-related chaperones), proteolysis (proteasome), and stabilization (compatible solute formation) to cope with polypeptide processing during thermal stress.
引用
收藏
页码:2365 / 2371
页数:7
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