Antibacterial activity of extract and fractions from branches of Protium spruceanum and cytotoxicity on fibroblasts

被引:11
作者
Amparo, Tatiane Roquete [1 ]
Rodrigues, Ivanildes Vasconcelos [2 ]
Seibert, Janaina Brandao [1 ]
Zaniti Souza, Rafaella Hilda [1 ]
de Oliveira, Amanda Ribeiro [1 ]
Rodrigues Cabral, Vivette Appolinario [3 ]
de Abreu Vieira, Paula Melo [4 ]
Brandao, Geraldo Celio [1 ]
Okuma, Adriana Akemi [5 ]
Vieira Filho, Sidney Augusto [1 ]
Medeirosteixeira, Luiz Fernando [1 ]
Bianco de Souza, Gustavo Henrique [1 ]
机构
[1] Univ Fed Ouro Preto, Dept Farm, Ouro Preto, Brazil
[2] Univ Fed Juiz de Fora, Dept Farm, Governador Valadares, Brazil
[3] Univ Fed Lavras, Dept Ciencias Florestais, Lavras, Brazil
[4] Univ Fed Ouro Preto, Dept Ciencias Biol, Ouro Preto, Brazil
[5] Ctr Fed Educ Tecnol Minas Gerais, Dept Quim, Belo Horizonte, MG, Brazil
关键词
Protium spruceanum; antbacterial activity; cytotoxicity; ANTIMICROBIAL ACTIVITY; ANTIFUNGAL ACTIVITY; BRAZIL; OILS;
D O I
10.1080/14786419.2017.1354182
中图分类号
O69 [应用化学];
学科分类号
070301 [无机化学];
摘要
The crude ethanol extract (CEE) and fractions from branches of Protium spruceanum were subjected to antibacterial and cytotoxicity assays. Compounds of the most active fraction were identified by GC-MS and LC-MS. CEE was active against 19 bacteria and the ethyl acetate fraction (EAF) showed the lowest minimum bactericidal concentration (MBC 0.3-80.0 mg/mL). Through time-kill assay was observed that EAF induced rapid bactericidal effect against Staphylococcus saprophyticus. The cytotoxicity tests against L929 fibroblasts showed great potential of EAF on the treatment of infections caused by five bacteria (MBC < IC50). The results provide in vitro scientific support to the possible application of branches of P. spruceanum as antimicrobial agent that may contribute for treatment of infections. [GRAPHICS] .
引用
收藏
页码:1951 / 1954
页数:4
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