Antibacterial activity of extract and fractions from branches of Protium spruceanum and cytotoxicity on fibroblasts

被引：11

作者：

Amparo, Tatiane Roquete ^{[1
]}

Rodrigues, Ivanildes Vasconcelos ^{[2
]}

Seibert, Janaina Brandao ^{[1
]}

Zaniti Souza, Rafaella Hilda ^{[1
]}

de Oliveira, Amanda Ribeiro ^{[1
]}

Rodrigues Cabral, Vivette Appolinario ^{[3
]}

de Abreu Vieira, Paula Melo ^{[4
]}

Brandao, Geraldo Celio ^{[1
]}

Okuma, Adriana Akemi ^{[5
]}

Vieira Filho, Sidney Augusto ^{[1
]}

Medeirosteixeira, Luiz Fernando ^{[1
]}

Bianco de Souza, Gustavo Henrique ^{[1
]}

机构：

[1] Univ Fed Ouro Preto, Dept Farm, Ouro Preto, Brazil

[2] Univ Fed Juiz de Fora, Dept Farm, Governador Valadares, Brazil

[3] Univ Fed Lavras, Dept Ciencias Florestais, Lavras, Brazil

[4] Univ Fed Ouro Preto, Dept Ciencias Biol, Ouro Preto, Brazil

[5] Ctr Fed Educ Tecnol Minas Gerais, Dept Quim, Belo Horizonte, MG, Brazil

来源：

NATURAL PRODUCT RESEARCH | 2018年 / 32卷 / 16期

关键词：

Protium spruceanum; antbacterial activity; cytotoxicity; ANTIMICROBIAL ACTIVITY; ANTIFUNGAL ACTIVITY; BRAZIL; OILS;

D O I：

10.1080/14786419.2017.1354182

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The crude ethanol extract (CEE) and fractions from branches of Protium spruceanum were subjected to antibacterial and cytotoxicity assays. Compounds of the most active fraction were identified by GC-MS and LC-MS. CEE was active against 19 bacteria and the ethyl acetate fraction (EAF) showed the lowest minimum bactericidal concentration (MBC 0.3-80.0 mg/mL). Through time-kill assay was observed that EAF induced rapid bactericidal effect against Staphylococcus saprophyticus. The cytotoxicity tests against L929 fibroblasts showed great potential of EAF on the treatment of infections caused by five bacteria (MBC < IC50). The results provide in vitro scientific support to the possible application of branches of P. spruceanum as antimicrobial agent that may contribute for treatment of infections. [GRAPHICS] .

引用

页码：1951 / 1954

页数：4

共 16 条

[1]

Antimicrobial and Selected In Vitro Enzyme Inhibitory Effects of Leaf Extracts, Flavonols and Indole Alkaloids Isolated from Croton menyharthii [J].

Aderogba, Mutalib A. ;

Ndhlala, Ashwell R. ;

Rengasamy, Kannan R. R. ;

Van Staden, Johannes .

MOLECULES, 2013, 18 (10) :12633-12644

[2]

Chemical constituents of Indonesian plant Protium java']javanicum Burm. f. and their antifeedant activities against Coptotermes formosanus Shiraki [J].

Adfa, Morina ;

Hattori, Yosuke ;

Ninomiya, Masayuki ;

Funahashi, Yasuhiro ;

Yoshimura, Tsuyoshi ;

Koketsu, Mamoru .

NATURAL PRODUCT RESEARCH, 2013, 27 (03) :270-273

[3]

Antifungal activity of phenolic compounds identified in flowers from North Eastern Portugal against Candida species [J].

Alves, Carlos Tiago ;

Ferreira, Isabel C. F. R. ;

Barros, Lillian ;

Silva, Sonia ;

Azeredo, Joana ;

Henriques, Mariana .

FUTURE MICROBIOLOGY, 2014, 9 (02) :139-146

[4]

Antifungal activity of the amyrin derivatives and in vitro inhibition of Candida albicans adhesion to human epithelial cells [J].

Johann, S. ;

Soldi, C. ;

Lyon, J. P. ;

Pizzolatti, M. G. ;

Resende, M. A. .

LETTERS IN APPLIED MICROBIOLOGY, 2007, 45 (02) :148-153

[5]

Pharmacodynamic studies of nitrofurantoin against common uropathogens [J].

Lindgren, P. Komp ;

Klockars, O. ;

Malmberg, C. ;

Cars, O. .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2015, 70 (04) :1076-1082

[6]

Antimicrobial and cytotoxic activity of red propolis: an alert for its safe use [J].

Lopez, B. G. C. ;

de Lourenco, C. C. ;

Alves, D. A. ;

Machado, D. ;

Lancellotti, M. ;

Sawaya, A. C. H. F. .

JOURNAL OF APPLIED MICROBIOLOGY, 2015, 119 (03) :677-687

[7]

Seasonal variation in the composition of the essential oils from the leaves, thin branches and resin of Protium spruceanum (Benth.) Engl. [J].

Machado, LB ;

Zoghbi, MD ;

Andrade, EHA .

FLAVOUR AND FRAGRANCE JOURNAL, 2003, 18 (04) :338-341

[8]

5-Methoxypropacin, a novel coumarinolignoid from Protium unifoliolatum [J].

Magalhaes, A ;

Zoghbi, MDB ;

Siani, AC .

NATURAL PRODUCT RESEARCH, 2006, 20 (01) :43-46

[9]

Toxicity of Fatty Acid Autoxidation Products: Highest Anti-Microbial Toxicity in the Initial Oxidative Phase [J].

Matikainen, Jorma ;

Lehtinen, Markku ;

Pelttari, Eila ;

Elo, Hannu .

MOLECULES, 2015, 20 (01) :35-42

[10]

Nascimento Paula F.C., 2007, Rev. bras. farmacogn., V17, P108, DOI 10.1590/S0102-695X2007000100020

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