The use of a recombinant lentiviral vector for ex vivo gene transfer into the rat CNS

被引：51

作者：

Englund, U

Ericson, C

Rosenblad, C

Mandel, RJ

Trono, D

Wictorin, K

Lundberg, C

机构：

[1] Lund Univ, Wallenberg Neurosci Ctr, Div Neurobiol, S-22362 Lund, Sweden

[2] Nsgene AS, Ballerup, Denmark

[3] Univ Geneva, Sch Med, CH-1211 Geneva, Switzerland

来源：

NEUROREPORT | 2000年 / 11卷 / 18期

关键词：

ex vivo gene transfer; green fluorescent protein; lentiviral vector;

D O I：

10.1097/00001756-200012180-00014

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A major obstacle in ex vivo gene transfer has been the loss of transgene expression soon after implantation of the grafted transduced cells. Recently, a lentiviral vector system has been developed which has proven to express high levels of transgenes in vivo after direct injection into the tissue. In this study, we have investigated the use of such a vector for ex vivo gene transfer to the brain. A number of neural cell types were found to be permissive to transduction by the lentiviral vector in vitro and a majority of them expressed the transgene after transplantation to the rat brain. Transgene expression was detected up to 8 weeks post-grafting. These findings suggest that recombinant lentiviral vectors may be used for further development of ex vivo gene therapy protocols to the CNS. NeuroReport 11:3973-3977 (C) 2000 Lippincott Williams & Wilkins.

引用

页码：3973 / 3977

页数：5

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