Inverted colloidal crystals as three-dimensional cell scaffolds

被引:130
作者
Kotov, NA
Liu, YF
Wang, SP
Cumming, C
Eghtedari, M
Vargas, G
Motamedi, M
Nichols, J
Cortiella, J
机构
[1] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA
[2] Oklahoma State Univ, Dept Chem, Stillwater, OK 74078 USA
[3] Nomad Inc, Stillwater, OK 74074 USA
[4] Univ Texas, Med Branch, Galveston, TX 77555 USA
关键词
D O I
10.1021/la049958o
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A new type of three-dimensional scaffold with inverted colloidal crystal geometry for the investigation of topological effects in cell cultures is introduced in this publication. The scaffolds are made by infiltration of the hexagonal crystal lattice of polystyrene spheres with sol-gel formulation and subsequent annealing. It possesses a relatively high degree of order among existing cell scaffolds and affords tight control over the scaffold porosity and tissue organization. The prepared scaffolds can be a convenient system for the investigation of cell-cell and cell-matrix interactions. Their biocompatibility is demonstrated for human hepatocellular carcinoma HEP G2 and human bone marrow HS-5 cell cultures. A preliminary effect of the scaffold topology on cell proliferation is observed. HEP G2 hepatocytes form a large number of 10-15 cell colonies on scaffolds made from 75-mum spheres, while their number diminishes for scaffolds from 10- and 160-mum spheres. Under similar conditions, HS-5 forms smaller colonies consisting of three to four cells in 90-mum cavities.
引用
收藏
页码:7887 / 7892
页数:6
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