Quantum dot-based sensitive detection of disease specific exosome in serum

被引:158
作者
Boriachek, Kseniia [1 ,2 ]
Islam, Md. Nazmul [1 ,2 ]
Gopalan, Vinod [3 ]
Lam, Alfred K. [3 ]
Nam-Trung Nguyen [2 ]
Shiddiky, Muhammad J. A. [1 ,2 ]
机构
[1] Griffith Univ, Sch Nat Sci, Nathan Campus, Nathan, Qld 4111, Australia
[2] Griffith Univ, Queensland Micro & Nanotechnol Ctr, Nathan Campus, Nathan, Qld 4111, Australia
[3] Griffith Univ, Menzies Hlth Inst Queensland, Canc Mol Pathol Lab, Sch Med, Gold Coast Campus, Southport, Qld 4222, Australia
基金
澳大利亚国家健康与医学研究理事会;
关键词
ELECTROCHEMICAL IMMUNOSENSOR; MEMBRANE-VESICLES; CANCER; BIOGENESIS; PARTICLE; SENSOR; CHIP;
D O I
10.1039/c7an00672a
中图分类号
O65 [分析化学];
学科分类号
070302 [分析化学];
摘要
Tumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific exosomes using quantum dots as signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by a generic tetraspanin antibody (e.g., CD9 or CD63) followed by the identification of disease-specific exosomes using cancer-related. Here, we used CdSe quantum dot (CdSeQD) functionalised-biotinylated HER-2 and FAM134B antibodies as breast and colon cancer markers. After magnetic washing and purification steps, acid dissolution of CdSeQDs and subsequent anodic stripping voltammetric quantification of Cd2+ were carried out at the bare glassy carbon working electrode. This method enabled sensitive detection of 100 exosomes per mu L with a relative standard deviation (% RSD) of < 5.5% in cancer cell lines and a small cohort of serum samples (n = 9) collected from patients with colorectal adenocarcinoma. We believe that our approach could potentially represent an effective bioassay for the quantification of disease-specific exosomes in clinical samples.
引用
收藏
页码:2211 / 2219
页数:9
相关论文
共 38 条
[1]
Tetraspanins in extracellular vesicle formation and function [J].
Andreu, Zoraida ;
Yanez-Mo, Maria .
FRONTIERS IN IMMUNOLOGY, 2014, 5
[2]
Exosomes in cancer development, metastasis, and drug resistance: a comprehensive review [J].
Azmi, Asfar S. ;
Bao, Bin ;
Sarkar, Fazlul H. .
CANCER AND METASTASIS REVIEWS, 2013, 32 (3-4) :623-642
[3]
Microfluidic isolation and transcriptome analysis of serum microvesicles [J].
Chen, Chihchen ;
Skog, Johan ;
Hsu, Chia-Hsien ;
Lessard, Ryan T. ;
Balaj, Leonora ;
Wurdinger, Thomas ;
Carter, Bob S. ;
Breakefield, Xandra O. ;
Toner, Mehmet ;
Irimia, Daniel .
LAB ON A CHIP, 2010, 10 (04) :505-511
[4]
de Vrij J, 2013, NANOMEDICINE-UK, V8, P1443, DOI [10.2217/NNM.12.173, 10.2217/nnm.12.173]
[5]
Electrochemical Sandwich Immunosensor for Determination of Exosomes Based on Surface Marker-Mediated Signal Amplification [J].
Doldan, Ximena ;
Fagundez, Pablo ;
Cayota, Alfonso ;
Laiz, Justo ;
Tosar, Juan Pablo .
ANALYTICAL CHEMISTRY, 2016, 88 (21) :10466-10473
[6]
Application of quantum dots as analytical tools in automated chemical analysis: A review [J].
Frigerio, Christian ;
Ribeiro, David S. M. ;
Rodrigues, S. Sofia M. ;
Abreu, Vera L. R. G. ;
Barbosa, Joao A. C. ;
Prior, Joao A. V. ;
Marques, Karine L. ;
Santos, Joao L. M. .
ANALYTICA CHIMICA ACTA, 2012, 735 :9-22
[7]
Nano-yeast-scFv probes on screen-printed gold electrodes for detection of Entamoeba histolytica antigens in a biological matrix [J].
Grewal, Yadveer S. ;
Shiddiky, Muhammad J. A. ;
Spadafora, Lauren J. ;
Cangelosi, Gerard A. ;
Trau, Matt .
BIOSENSORS & BIOELECTRONICS, 2014, 55 :417-422
[8]
Integrated immunoisolation and protein analysis of circulating exosomes using microfluidic technology [J].
He, Mei ;
Crow, Jennifer ;
Roth, Marc ;
Zeng, Yong ;
Godwin, Andrew K. .
LAB ON A CHIP, 2014, 14 (19) :3773-3780
[9]
Microarray detection of novel nuclear RNA substrates for the exosome [J].
Houalla, Rynn ;
Devaux, Frdric ;
Fatica, Alessandro ;
Kufel, Joanna ;
Barrass, David ;
Torchet, Claire ;
Tollervey, David .
YEAST, 2006, 23 (06) :439-454
[10]
Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor [J].
Im, Hyungsoon ;
Shao, Huilin ;
Park, Yong Il ;
Peterson, Vanessa M. ;
Castro, Cesar M. ;
Weissleder, Ralph ;
Lee, Hakho .
NATURE BIOTECHNOLOGY, 2014, 32 (05) :490-U219