Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511

被引：401

作者：

Rodin, Sergey ^{[1
]}

Domogatskaya, Anna ^{[1
]}

Strom, Susanne ^{[2
,3
]}

Hansson, Emil M. ^{[4
]}

Chien, Kenneth R. ^{[4
,5
]}

Inzunza, Jose ^{[6
,7
]}

Hovatta, Outi ^{[2
,3
]}

Tryggvason, Karl ^{[1
]}

机构：

[1] Karolinska Inst, Div Matrix Biol, Dept Med Biochem & Biophys, Stockholm, Sweden

[2] Karolinska Inst, Div Obstet & Gynecol, Dept Clin Sci Intervent & Technol, Stockholm, Sweden

[3] Karolinska Univ Hosp, Stockholm, Sweden

[4] Massachusetts Gen Hosp, Cardiovasc Res Ctr, Boston, MA 02114 USA

[5] Dept Stem Cell & Regenerat Biol, Cambridge, MA USA

[6] Karolinska Inst, Novum, Dept Biosci & Nutr, Huddinge, Sweden

[7] Karolinska Hosp, S-10401 Stockholm, Sweden

来源：

NATURE BIOTECHNOLOGY | 2010年 / 28卷 / 06期

基金：

瑞典研究理事会;

关键词：

EXPRESSION; DERIVATION; PURIFICATION; FIBROBLASTS; CULTURE; SYSTEM; GROWTH; CHAIN; LINES;

D O I：

10.1038/nbt.1620

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a system for culturing human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells on a recombinant form of human laminin-511, a component of the natural hES cell niche. The system is devoid of animal products and feeder cells and contains only one undefined component, human albumin. The hES cells self-renewed with normal karyotype for at least 4 months (20 passages), after which the cells could produce teratomas containing cell lineages of all three germ layers. When plated on laminin-511 in small clumps, hES cells spread out in a monolayer, maintaining cellular homogeneity with approximately 97% OCT4-positive cells. Adhesion of hES cells was dependent on alpha 6 beta 1 integrin. The use of homogeneous monolayer hES or iPS cell cultures provides more controllable conditions for the design of differentiation methods. This xeno-free and feeder-free system may be useful for the development of cell lineages for therapeutic purposes.

引用

页码：611 / U102

页数：7

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