Homogeneous electrogenerated chemiluminescence immunoassay using a luminol-labeled digoxin hapten

被引：10

作者：

Zhang, CX ^{[1
]}

Zhang, HH ^{[1
]}

Feng, ML ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Sch Chem & Mat Sci, Dept Chem, Xian 710062, Peoples R China

来源：

ANALYTICAL LETTERS | 2003年 / 36卷 / 06期

基金：

中国国家自然科学基金;

关键词：

electrogenerated chemiluminescence; immunoassay; digoxin; luminol;

D O I：

10.1081/AL-120020146

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel homogeneous electrogenerated chemiluminescence (ECL) immunoassay for digoxin antibody and digoxin hapten was developed using a luminol-labeled digoxin hapten. ECL emission was electrochemically generated from the luminol-labeled digoxin and decreased markedly in the presence of anti-digoxin antibody due to steric hindrance. The anti-digoxin antibody concentration was determined in the range from 115000 to 11500 dilution. Digoxin hapten was determined throughout its therapeutic range with a detection limit of 0.10ng/mL and in a three orders of magnitude range by competitive immunoreaction between luminol-labeled digoxin and digoxin with fixed quantity of anti-digoxin antibody. The proposed method has been applied to determine anti-digoxin antibody and digoxin in human control serum with satisfactory results.

引用

页码：1103 / 1114

页数：12

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