Pestalotiopsis guepinii, a taxol-producing endophyte of the Wollemi pine, Wollemia nobilis

被引:98
作者
Strobel, GA [1 ]
Hess, WM
Li, JY
Ford, E
Sears, J
Sidhu, RS
Summerell, B
机构
[1] Montana State Univ, Dept Plant Pathol, Bozeman, MT 59717 USA
[2] Brigham Young Univ, Dept Bot & Range Sci, Provo, UT 84601 USA
[3] Montana State Univ, Dept Chem, Bozeman, MT 59717 USA
[4] Cytoclonal Pharmaceut Inc, Dallas, TX 75235 USA
[5] Royal Bot Gardens, Sydney, NSW 2000, Australia
关键词
D O I
10.1071/BT96094
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Wollemia nobilis (Wollemi pine), an araucariaceous plant, whose closest known relatives are from the Jurassic period, occurs in the Wollemi National Park near Sydney, Australia. This tree is host to many endophytic fungi, including Pestalotiopsis guepinii which produces taxol, an important anticancer drug. It was shown by immunological, spectroscopic and chromatographic means to be identical with authentic taxol obtained from Tarus brevifolia (Pacific yew). Since the Wollemi pine does not produce taxol, how might a taxol-producing fungus be present in this unusual tree? Spores of P. guepinii possess several appendages which strongly interact with hydrophobic surfaces including plastics and the pinnae of birds' feathers. Scanning electron microscopy of the conidia of P. guepinii clearly shows the spores on the feathers of a green-checked conure and a cockatiel. Conceivably, the fungus may have acquired the ability to produce taxol from a foreign or local yew and then is carried to the Wollemi pine site by native birds.
引用
收藏
页码:1073 / 1082
页数:10
相关论文
共 17 条
[1]   HPLC SEPARATION OF TAXOL AND CEPHALOMANNINE [J].
CARDELLINA, JH .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1991, 14 (04) :659-665
[2]   AN ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF TAXOL AND TAXANES IN TAXUS SP TISSUES AND HUMAN PLASMA [J].
GROTHAUS, PG ;
RAYBOULD, TJG ;
BIGNAMI, GS ;
LAZO, CB ;
BYRNES, JB .
JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 158 (01) :5-15
[3]  
HAWKSWORTH DL, 1974, MYCOLOGISTS HDB
[4]  
Jones W. G., 1995, Telopea, V6, P173
[5]   Endophytic taxol-producing fungi from bald cypress, Taxodium distichum [J].
Li, JY ;
Strobel, G ;
Sidhu, R ;
Hess, WM ;
Ford, EJ .
MICROBIOLOGY-UK, 1996, 142 :2223-2226
[6]  
NELSON TA, 1983, FUSARIUM SPECIES
[7]  
PUROHIT DK, 1989, CURR SCI INDIA, V58, P659
[8]   TAXOL AND TAXANE PRODUCTION BY TAXOMYCES-ANDREANAE, AN ENDOPHYTIC FUNGUS OF PACIFIC YEW [J].
STIERLE, A ;
STROBEL, G ;
STIERLE, D .
SCIENCE, 1993, 260 (5105) :214-216
[9]   Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana [J].
Strobel, G ;
Yang, XS ;
Sears, J ;
Kramer, R ;
Sidhu, RS ;
Hess, WM .
MICROBIOLOGY-UK, 1996, 142 :435-440
[10]  
STROBEL GA, 1996, IN PRESS J IND MICRO