Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis-mediated cell death

被引：440

作者：

Gupta, Anurag Kumar ^{[1
]}

Joshi, Manjunath B. ^{[2
]}

Philippova, Maria ^{[2
]}

Erne, Paul ^{[3
]}

Hasler, Paul ^{[4
]}

Hahn, Sinuhe ^{[1
]}

Resink, Therese J. ^{[2
]}

机构：

[1] Univ Basel Hosp, Lab Prenatal Med, Dept Biomed, Univ Womens Hosp, CH-4031 Basel, Switzerland

[2] Univ Basel Hosp, Lab Signal Transduct, Dept Biomed, CH-4031 Basel, Switzerland

[3] Kantonsspital Luzern, Dept Cardiol, Luzern, Switzerland

[4] Kantonsspital Aarau, Dept Rheumatol, Aarau, Switzerland

来源：

FEBS LETTERS | 2010年 / 584卷 / 14期

基金：

瑞士国家科学基金会;

关键词：

Neutrophil; Neutrophil extracellular trap; Endothelial cell; TRANSMIGRATION; STREPTOCOCCUS; PREECLAMPSIA; ESCAPE; ALLOWS; LEADS; NETS;

D O I：

10.1016/j.febslet.2010.06.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neutrophil interaction with activated endothelial cells (EC) is required for transmigration. We examined consequences of this interaction on NETosis. Co-culture of activated EC with neutrophils induced neutrophil extracellular trap (NET) formation, which was partially dependent on production of IL-8 by activated EC. Extended neutophil/EC co-culture resulted in EC damage, which could be abrogated by inclusion of either diphenyleneiodonium to inhibit the NAPDH oxidase pathway required for NETosis, or DNAse to disrupt NETs. These findings offer new insight into mechanisms whereby NETs trigger damage to the endothelium in sepsis, small vessel vasculitis and possibly the villous trophoblast in preeclampsia. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3193 / 3197

页数：5

共 22 条

[1] An endonuclease allows Streptococcus pneumoniae to escape from neutrophil extracellular traps [J].

Beiter, K ;

Wartha, F ;

Albiger, B ;

Normark, S ;

Zychlinsky, A ;

Henriques-Normark, B .

CURRENT BIOLOGY, 2006, 16 (04) :401-407

[2] Restoration of NET formation by gene therapy in CGD controls aspergillosis [J].

Bianchi, Matteo ;

Hakkim, Abdul ;

Brinkmann, Volker ;

Siler, Ulrich ;

Seger, Reinhard A. ;

Zychlinsky, Arturo ;

Reichenbach, Janine .

BLOOD, 2009, 114 (13) :2619-2622

[3] Neutrophil extracellular traps kill bacteria [J].

Brinkmann, V ;

Reichard, U ;

Goosmann, C ;

Fauler, B ;

Uhlemann, Y ;

Weiss, DS ;

Weinrauch, Y ;

Zychlinsky, A .

SCIENCE, 2004, 303 (5663) :1532-1535

[4] Beneficial suicide: why neutrophils die to make NETs [J].

Brinkmann, Volker ;

Zychlinsky, Arturo .

NATURE REVIEWS MICROBIOLOGY, 2007, 5 (08) :577-582

[5] DNase expression allows the pathogen group A streptococcus to escape killing in neutrophil extracellular traps [J].

Buchanan, JT ;

Simpson, AJ ;

Aziz, RK ;

Liu, GY ;

Kristian, SA ;

Kotb, M ;

Feramisco, J ;

Nizet, V .

CURRENT BIOLOGY, 2006, 16 (04) :396-400

[6] Platelet TLR4 activates neutrophil extracellular traps to ensnare bacteria in septic blood [J].

Clark, Stephen R. ;

Ma, Adrienne C. ;

Tavener, Samantha A. ;

McDonald, Braedon ;

Goodarzi, Zahra ;

Kelly, Margaret M. ;

Patel, Kamala D. ;

Chakrabarti, Subhadeep ;

McAvoy, Erin ;

Sinclair, Gary D. ;

Keys, Elizabeth M. ;

Allen-Vercoe, Emma ;

DeVinney, Rebekah ;

Doig, Christopher J. ;

Green, Francis H. Y. ;

Kubes, Paul .

NATURE MEDICINE, 2007, 13 (04) :463-469

[7]

Ermert David, 2009, V470, P293, DOI 10.1007/978-1-59745-204-5_21

[8] Novel cell death program leads to neutrophil extracellular traps [J].

Fuchs, Tobias A. ;

Abed, Ulrike ;

Goosmann, Christian ;

Hurwitz, Robert ;

Schulze, Ilka ;

Wahn, Volker ;

Weinrauch, Yvette ;

Brinkmann, Volker ;

Zychlinsky, Arturo .

JOURNAL OF CELL BIOLOGY, 2007, 176 (02) :231-241

[9] Leishmania amazonensis promastigotes induce and are killed by neutrophil extracellular traps [J].

Guimaraes-Costa, Anderson B. ;

Nascimento, Michelle T. C. ;

Froment, Giselle S. ;

Soares, Rodrigo P. P. ;

Morgado, Fernanda N. ;

Conceicao-Silva, Fatima ;

Saraiva, Elvira M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (16) :6748-6753

[10] Induction of neutrophil extracellular DNA lattices by placental microparticles and IL-8 and their presence in preeclampsia [J].

Gupta, AK ;

Hasler, P ;

Holzgreve, W ;

Gebhardt, S ;

Hahn, S .

HUMAN IMMUNOLOGY, 2005, 66 (11) :1146-1154

← 1 2 3 →