GFP expression alters osteosarcoma cell biology

被引：15

作者：

Dass, Crispin R. ^{[1
]}

Choong, Peter F.

机构：

[1] Univ Melbourne, St Vincent Hosp, Dept Orthopaed, Fitzroy, Vic 3065, Australia

[2] Peter MacCallum Canc Inst, Bone & Soft Tissue Sarcoma Serv, Melbourne, Vic 3000, Australia

来源：

DNA AND CELL BIOLOGY | 2007年 / 26卷 / 08期

关键词：

D O I：

10.1089/dna.2006.0531

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Green fluorescent protein (GFP) expression is widely used for tracking of cells in vivo. Three human osteosarcoma cell lines were transfected with GFP and analyzed for expression of tissue markers and physiology. GFP-mediated increased tumor aggression in vivo in one cell line was corroborated by altered expression of bone markers and osteoblast-like cell biology in vitro as a result of transfection. The use of GFP for cell tracking in osteosarcoma animal models should therefore be used with caution.

引用

页码：599 / 601

页数：3

共 9 条

[1] GFP expression in muscle cells impairs actin-myosin interactions:: implications for cell therapy [J].

Agbulut, O ;

Coirault, C ;

Niederländer, N ;

Huet, A ;

Vicart, P ;

Hagège, A ;

Puceat, M ;

Menasché, P .

NATURE METHODS, 2006, 3 (05) :331-331

[2] Malignant reversion of a human osteosarcoma cell line, Saos-2, by inhibition of NFκB [J].

Andela, VB ;

Sheu, TJ ;

Puzas, EJ ;

Schwarz, EM ;

O'Keefe, RJ ;

Rosier, RN .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 297 (02) :237-241

[3] Transgenic monkeys produced by retroviral gene transfer into mature oocytes [J].

Chan, AWS ;

Chong, KY ;

Martinovich, C ;

Simerly, C ;

Schatten, G .

SCIENCE, 2001, 291 (5502) :309-312

[4] Down-regulation of osteoblastic cell differentiation by epidermal growth factor receptor [J].

Chien, HH ;

Lin, WL ;

Cho, MI .

CALCIFIED TISSUE INTERNATIONAL, 2000, 67 (02) :141-150

[5] Downregulation of uPAR confirms link in growth and metastasis of osteosarcoma [J].

Dass, Crispin R. ;

Nadesapillai, Anne P. W. ;

Robin, Daniel ;

Howard, Monique L. ;

Fisher, Jane L. ;

Zhou, Hong ;

Choong, Peter F. M. .

CLINICAL & EXPERIMENTAL METASTASIS, 2005, 22 (08) :643-652

[6] Osteoblast-related gene expression of bone marrow cells during the osteoblastic differentiation induced by type I collagen [J].

Mizuno, M ;

Kuboki, Y .

JOURNAL OF BIOCHEMISTRY, 2001, 129 (01) :133-138

[7] Prostate-specific antigen modulates genes involved in bone remodeling and induces osteoblast differentiation of human osteosarcoma cell line SaOS-2 [J].

Nadiminty, N ;

Lou, W ;

Lee, SO ;

Mehraein-Ghomi, F ;

Kirk, JS ;

Conroy, JM ;

Zhang, HT ;

Gao, AC .

CLINICAL CANCER RESEARCH, 2006, 12 (05) :1420-1430

[8] Responses of vascular endothelial cells to angiogenic signaling are important for tumor cell survival [J].

Shan, SQ ;

Robson, ND ;

Cao, YT ;

Qiao, T ;

Li, CY ;

Kontos, CD ;

Garcia-Blanco, M ;

Dewhirst, MW .

FASEB JOURNAL, 2003, 17 (15) :326-+

[9] Inhibition of cell migration, spreading, and focal adhesions by tumor suppressor PTEN [J].

Tamura, M ;

Gu, JG ;

Matsumoto, K ;

Aota, S ;

Parsons, R ;

Yamada, KM .

SCIENCE, 1998, 280 (5369) :1614-1617

← 1 →