Capillary zone electrophoresis for the separation and characterization of humic acids

被引:42
作者
Fetsch, D [1 ]
Havel, J [1 ]
机构
[1] Masaryk Univ, Fac Sci, Dept Analyt Chem, CS-61137 Brno, Czech Republic
关键词
buffer composition; humic acids;
D O I
10.1016/S0021-9673(97)01116-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Capillary zone electrophoresis (CZE) was used for the separation and characterization of humic acids (HAs) of different origin. UV-Vis detection and/or fast scanning of spectra during the separation was applied for the detection. From several background electrolytes (BGEs) studied, it was found that especially those containing amino acids are the most suitable and led re, the separation of HAs into several fractions. The composition and pH of buffers were optimized. Up to 30 fractions were separated using a high boric acid concentration (350 mM) BGE. The results can be used as real "fingerprints" to characterize HAs of different origin. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:189 / 202
页数:14
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