Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens

被引：86

作者：

Nauerby, B ^{[1
]}

Pedersen, K ^{[1
]}

Madsen, M ^{[1
]}

机构：

[1] Danish Vet Inst, Dept Poultry Fish & Fur Anim, DK-8200 Aarhus N, Denmark

来源：

VETERINARY MICROBIOLOGY | 2003年 / 94卷 / 03期

关键词：

Clostridium perfringens; chicken; pulsed-field gel electrophoresis;

D O I：

10.1016/S0378-1135(03)00118-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of this study was to analyse the genetic diversity among Clostridium perfringens isolates from Danish broiler chickens since both sick and presumably healthy animals were investigated. Isolates (n = 279) collected from chickens from 25 farms were analysed by pulsed-field gel electrophoresis (PFGE) with the restriction enzyme SmaI. A high genetic diversity was found. Isolates with different PFGE types were toxin typed by PCR and all were found to be of type A. The results showed that healthy broiler chickens carried several different C. perfringens clones both within a flock and even within individual birds, whereas flocks suffering from necrotic enteritis (NE) or cholangio-hepatitis carried only one or two clones. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：257 / 266

页数：10

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