The TLR4+896 polymorphism is not associated with lipopolysaccharide hypo-responsiveness in leukocytes

被引：27

作者：

Imahara, SD ^{[1
]}

Jelacic, S ^{[1
]}

Junker, CE ^{[1
]}

O'Keefe, GE ^{[1
]}

机构：

[1] Univ Washington, Harborview Med Ctr, Dept Surg, Seattle, WA 98104 USA

来源：

GENES AND IMMUNITY | 2005年 / 6卷 / 01期

关键词：

monocyte; polymorphism; TLR4; p38; cytokines; endotoxin;

D O I：

10.1038/sj.gene.6364147

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Toll- like receptor 4 ( TLR- 4) is required for detection of Gram negative bacterial infections by binding lipopolysaccharide ( LPS) and for the initiation of inflammatory signaling. Recent studies have demonstrated that a nonsynonymous single- nucleotide polymorphism ( Asp299Gly, A+896G) is associated with decreased endotoxin responsiveness and poor outcomes from sepsis. We show that human carriers of this polymorphism show no deficit in LPS induced peripheral blood mononuclear cell ( PBMC) mitogen- activated protein kinase ( MAPK) activity, no reduction in sensitivity to endotoxin, and variable differences in whole- blood inflammatory cytokine production. These results indicate that this mutation is not a primary determinant of human endotoxin sensitivity.

引用

页码：37 / 43

页数：7

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