Chrysanthemum morifolium Ramat. reduces the oxidized LDL-induced expression of intercellular adhesion molecule-1 and E-selectin in human umbilical vein endothelial cells

被引：86

作者：

Lii, Chong-Kuei ^{[1
]}

Lei, Yen-Ping ^{[2
]}

Yao, Hsien-Tsung ^{[1
]}

Hsieh, Yun-Sheng ^{[2
]}

Tsai, Chia-Wen ^{[1
]}

Liu, Kai-Li ^{[2
,3
]}

Chen, Haw-Wen ^{[1
]}

机构：

[1] China Med Univ, Dept Nutr, Taichung 404, Taiwan

[2] Chung Shan Med Univ, Dept Nutr, Taichung 402, Taiwan

[3] Chung Shan Med Univ Hosp, Dept Dietitian, Taichung 402, Taiwan

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2010年 / 128卷 / 01期

关键词：

Chrysanthemum morifolium Ramat; E-selectin; Human umbilical vein endothelial cells (HUVECs); Intercellular adhesion molecule-1 (ICAM-1); Oxidized LDL (oxLDL); LOW-DENSITY-LIPOPROTEIN; KAPPA-B; COMPOSITAE PLANTS; MONOCYTE ADHESION; HEART-DISEASE; FLAVONOIDS; ALPHA; IDENTIFICATION; ACTIVATION; REDUCTION;

D O I：

10.1016/j.jep.2010.01.018

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Ethnopharmacological relevance: The flower of Chrysanthemum morifolium Ramat. (CM) with antioxidant, cardiovascular protective and anti-inflammatory functions, has been widely used in China for hundreds of years as a healthy beverage and medicine. Aims of the study: The purpose of the present study is to investigate the effects of HCM (a hot water extract of the flower of Chrysanthemum morifolium Ramat. [CM]), ECM (an ethanol extract of CM), and the abundant flavonoids apigenin and luteolin in CM on the oxidized LDL (oxLDL)-induced expression of ICAM-1 and E-selectin in human umbilical vein endothelial cells (HUVECs). The possible mechanism of these effects was also determined. Materials and methods: MTT assay was for cell viability. Western blot was used for ICAM-1 and E-selection protein expression, and for activation of protein kinase B (PKB) and cAMP responsive element binding protein (CREB) proteins. Fluorescence flow cytometry was for ICAM-1 and E-selectin expression on cell surface. DCF-DA flow cytometric assay was used for reactive oxygen species (ROS) production. Results: HCM, ECM, apigenin, and luteolin dose-dependently inhibited ICAM-1 and E-selectin expression and adhesion of HL-60 by oxLDL. HCM, ECM, apigenin, and luteolin reversed the inhibition of phosphorylation of Akt and CREB by oxLDL; however, this reversion was abolished by wortmannin. In addition, wortmannin abrogated the inhibitory effects of CM extracts, apigenin and luteolin on adhesion molecule expression. The ROS scavenging capability of HCM, ECM, apigenin, and luteolin proceeded dose-dependently in the presence of oxLDL. Conclusion: CM is a plant with cardiovascular-protective potential and the inhibitory effects of CM on ICAM-1 and E-selectin expression are, at least partially, attributed to its antioxidant activity and modulation of the PI3K/Akt signaling pathway. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：213 / 220

页数：8

共 41 条

[1]

MODIFIED FORMS OF LOW-DENSITY-LIPOPROTEIN AND ATHEROSCLEROSIS [J].