Identification of a resistance gene Rpi-dlc1 to Phytophthora infestans in European accessions of Solanum dulcamara

被引：30

作者：

Golas, Tomasz Michal ^{[1
,2
,6
]}

Sikkema, Anne ^{[5
]}

Gros, Jack ^{[5
]}

Feron, Richard M. C. ^{[1
,2
]}

van den Berg, Ronald G. ^{[3
,6
]}

van der Weerden, Gerard M. ^{[4
,6
]}

Mariani, Celestina ^{[2
,6
]}

Allefs, J. J. H. M. ^{[5
,6
]}

机构：

[1] Radboud Univ Nijmegen, Plant Cell Biol Grp, NL-6525 ED Nijmegen, Netherlands

[2] Radboud Univ Nijmegen, Inst Water & Wetlands Res, Dept Expt Bot, NL-6525 AJ Nijmegen, Netherlands

[3] Wageningen Univ, Biosystemat Grp, NL-6703 BL Wageningen, Netherlands

[4] Radboud Univ Nijmegen, Inst Water & Wetlands Res Bot & Expt Garden, NL-6525 ED Nijmegen, Netherlands

[5] Agr Res, NL-8314 PP Bant, Netherlands

[6] CBSG, NL-6700 AB Wageningen, Netherlands

来源：

THEORETICAL AND APPLIED GENETICS | 2010年 / 120卷 / 04期

关键词：

LATE-BLIGHT RESISTANCE; BROAD-SPECTRUM RESISTANCE; RACE-SPECIFIC RESISTANCE; POTATO LATE BLIGHT; R-GENE; DISEASE-RESISTANCE; CHROMOSOME-IX; BULBOCASTANUM; TOMATO; LOCUS;

D O I：

10.1007/s00122-009-1202-3

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Initial screening of 14 Solanum dulcamara accessions enabled the identification of individuals resistant and susceptible to Phytophthora infestans. Crosses between contrasting genotypes resulted in three F(2)-BC(1) populations segregating for resistance to late blight in a laboratory assay and under field conditions. Genetic profiling of one of these populations using 128 AFLP primers generated three markers linked to the resistant phenotype. Blast analysis of the sequenced markers resulted in a plausible gene position on the distal end of the long arm of chromosome 9 that could be confirmed by CAPS markers. Thus, we describe a first resistant gene, named Rpi-dlc1, from S. dulcamara, a Solanum species native to Europe. In addition, one population was tested for broadness of resistance responses using a set of seven additional P. infestans isolates, varying in virulence. This indicated the possible presence of additional Rpi genes.

引用

页码：797 / 808

页数：12

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