Differentiation of wine lactic acid bacteria species based on RFLP analysis of a partial sequence of rpoB gene

被引：18

作者：

Claisse, Olivier ^{[1
]}

Renouf, Vincent ^{[1
]}

Lonvaud-Funel, Aline ^{[1
]}

机构：

[1] Univ Bordeaux 2, INRA, ISVV, UMR Oenol, F-33405 Talence, France

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 69卷 / 02期

关键词：

lactic acid bacteria; rpoB; differentiation; PCR-RFLP;

D O I：

10.1016/j.mimet.2007.01.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

PCR-R-FLP analysis of rpoB sequences were used to identify lactic acid bacteria (LAB) species commonly isolated from wine. Strains of seven cocci and 12 lactobacilli species could be identified after single digestion with AciI for the cocci and two or three digestions (AciI, HinfI and MseI) for the rods and preceded by colonies isolation on solid selective medium and microscope observation to distinguish cocci and rods cells. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：387 / 390

页数：4

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