Oligogalacturonides stimulate pericycle cell wall thickening and cell divisions leading to stoma formation in tobacco leaf explants

Novel developmental events induced by micromolar concentrations of oligogalacturonides (OGs) in tobacco leaf explants cultured in vitro are described. Oligogalacturonides induced acceleration and synchronization of the mitotic activity of guard-cell precursors in the epidermis. In explants cultured for 24 h in the presence of OGs, the number of stomatal mitoses was higher than that observed in explants cultured in the absence of OGs; however, at the end of the culture period the density of mature stomata did not vary upon OG treatment. The OG-induced activation of stomatal mitosis was reduced by exogenously added indole-3-acetic acid (IAA). Oligogalacturonides also enhanced mean wall thickness, mainly due to cellulose deposition, of foliar pericycle cells, as well as the number of extra-thick-walled pericycle cells; the pericycle thus formed a sheath surrounding phloem and xylem. Indole-3-acetic acid decreased the number of extra-thick-walled cells forming in the presence of OGs but did not influence wall thickness. Moreover: OGs inhibited the stimulation of mitotic activity of phloem parenchyma cells (vascular mitoses) induced by auxin, leading to a nearly complete inhibition of IAA-induced formation of callus and of meristemoids of indirect origin. Instead, OGs did not influence mitotic activity occurring in the absence of auxin. All in all, our results provide further evidence of the pleiotropic role exerted on plant development by these oligosaccharins, and of the antagonism between auxin and OGs.