Detection of plum bark necrosis stem pitting-associated virus (PBNSPaV) from different stone fruit species and optimisation of diagnostic tools

被引：6

作者：

Amenduni, T ^{[1
]}

Hobeika, C ^{[1
]}

Minafra, A ^{[1
]}

Savino, V ^{[1
]}

机构：

[1] Univ Bari, Dipartimento Protez Piante & Microbiol Applicata, I-70126 Bari, Italy

来源：

PROCEEDINGS OF THE XIXTH INTERNATIONAL SYMPOSIUM ON VIRUS AND VIRUS-LIKE DISEASES OF TEMPERATE FRUIT CROPS: FRUIT TREE DISEASES | 2004年 / 657期

关键词：

PBNSPaV; stem pitting; stone fruits;

D O I：

10.17660/ActaHortic.2004.657.10

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Stone fruit trees, showing clear-cut symptoms of stem pitting, were assayed by RT-PCR for the presence of PBNSPaV. A DNA fragment of the expected size (290bp) was amplified from total nucleic acid extracts from cortical scrapings of almond 'Filippo Ceo', peach 'Springcrest' and 'May Crest', plum 'Black Beaut', apricot 'Castelbrite' and 'Tyrinthos', and sweet cherry 'Ferrovia'. The test was repeated monthly throughout year using different tissues (flowers, leaves, dormant phloem) to identify the optimal sampling period and tissue type for reliable diagnosis. Different protocols for total nucleic acid extraction and RT-PCR amplification were compared.

引用

页码：93 / 97

页数：5

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