The mt1 melatonin receptor and RORβ receptor are co-localized in specific TSH-immunoreactive cells in the pars tuberalis of the rat pituitary

被引：101

作者：

Klosen, P ^{[1
]}

Bienvenu, C ^{[1
]}

Demarteau, O ^{[1
]}

Dardente, H ^{[1
]}

Guerrero, H ^{[1
]}

Pévet, P ^{[1
]}

Masson-Pévet, M ^{[1
]}

机构：

[1] Univ Strasbourg, CNRS, UMR 7518, F-67000 Strasbourg, France

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2002年 / 50卷 / 12期

关键词：

mt1 melatonin receptor; ROR beta receptor; pituitary; pars tuberalis; in situ hybridization; immunocytochemistry;

D O I：

10.1177/002215540205001209

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The pars tuberalis (PT) of the pituitary represents an important target site for the time-pacing pineal hormone melatonin because it expresses a large number of mt1 receptors. Functional studies suggest that the PT mediates the seasonal effects of melatonin on prolactin (PRL) secretion. The aim of this study was the characterization of the phenotype of melatonin-responsive cells. Furthermore, we determined whether RORbeta, a retinoid orphan receptor present in the PT, was co-expressed in the same cells. We combined nonradioactive in situ hybridization (ISH) with hapten-labeled riboprobes for detection of the receptors and immunocytochemistry (ICC) for detection of alphaGSU (alpha-glycoprotein subunit), betaTSH, betaFSH, betaLH, GH, PRL, and ACTH. Expression of mt1 mRNA was found in small round cells, co-localized with alphaGSU and betaTSH. However, not all betaTSH-containing cells expressed mtl mRNA. The distribution of mt1- and RORbeta-positive cells appeared to overlap, although more cells were labeled for RORbeta than for mtl. Gonadotrophs, as well as other pars distalis cell types, were never labeled for mtl melatonin receptor. Therefore, this study identifies the "specific" cells of the PT as the mt1 melatonin receptor-expressing cells.

引用

页码：1647 / 1657

页数：11

共 72 条

[1] Disruption of retinoid-related orphan receptor β changes circadian behavior, causes retinal degeneration and leads to vacillans phenotype in mice [J].