Purification, characterization and developmental expression of pig liver PSP

被引：7

作者：

Kaneki, K ^{[1
]}

Matsumoto, M ^{[1
]}

Suzuki, K ^{[1
]}

Akuzawa, M ^{[1
]}

Oka, T ^{[1
]}

机构：

[1] Kagoshima Univ, Fac Agr, Dept Vet Med, Kagoshima 8900065, Japan

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2003年 / 134卷 / 04期

关键词：

pig; perchloric acid-soluble protein; development; ACID-SOLUBLE-PROTEIN; DIFFERENTIATION; INHIBITOR;

D O I：

10.1016/S1096-4959(02)00269-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a perchloric acid-soluble protein designated as PL-PSP from the post-mitochondria supernatant fraction of pig liver. It is soluble in 5% perchloric acid and purified by ammonium sulfate fractionation and CM-Sephadex chromatography. The PL-PSP showed approximately 80-90% homology with PSP isolated from rat liver (RL-PSP) with its partial amino acid sequences. The protein has a molecular mass of approximately 14 kDa which was slightly higher than that of RL-PSR It inhibited protein synthesis in a rabbit reticulocyte lysate system. The expression of PL-PSP was predominant in liver, kidney and duodenum, and was also expressed in stomach, lung and brain. PL-PSP expression in liver increased from the 1st day to the 1st month. Thus, our findings are the first report on the presence of a PSP in porcine tissues which may be involved in the regulation of cellular growth and differentiation. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：571 / 578

页数：8

共 12 条

[1] Purification, characterization and differentiation-dependent expression of a perchloric acid soluble protein from rat kidney [J].

Asagi, K ;

Oka, T ;

Arao, K ;

Suzuki, I ;

Thakur, MK ;

Izumi, K ;

Natori, Y .

NEPHRON, 1998, 79 (01) :80-90

[2] The primary structure of UK114 tumor antigen [J].

Ceciliani, F ;

Faotto, L ;

Negri, A ;

Colombo, I ;

Berra, B ;

Bartorelli, A ;

Ronchi, S .

FEBS LETTERS, 1996, 393 (2-3) :147-150

[3] Expression and cellular distribution of perchloric acid-soluble protein is dependent on the cell-proliferating states of NRK-52E cells [J].

Kanouchi, H ;

Oka, T ;

Asagi, K ;

Tachibana, H ;

Yamada, K .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2000, 57 (07) :1103-1108

[4] Recombinant expression of perchloric acid-soluble protein reduces cell proliferation [J].

Kanouchi, H ;

Tachibana, H ;

Oka, T ;

Yamada, K .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2001, 58 (09) :1340-1343

[5] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[6] Ribonuclease activity of rat liver perchloric acid-soluble protein, a potent inhibitor of protein synthesis [J].

Morishita, R ;

Kawagoshi, A ;

Sawasaki, T ;

Madin, K ;

Ogasawara, T ;

Oka, T ;

Endo, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (29) :20688-20692

[7] Purification, characterization and developmental expression of chick (Gallus domesticus) liver PSP protein [J].

Nordin, H ;

Matsumoto, M ;

Suzuki, K ;

Kaneki, K ;

Natori, Y ;

Kishi, K ;

Oka, T .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2001, 128 (01) :135-143

[8]

OKA T, 1995, J BIOL CHEM, V270, P30060, DOI 10.1074/jbc.270.50.30060

[9] Hrp12, a novel heat-responsive, tissue-specific, phosphorylated protein isolated from mouse liver [J].

Samuel, SJ ;

Tzung, SP ;

Cohen, SA .

HEPATOLOGY, 1997, 25 (05) :1213-1222

[10] Isolation and characterization of a 14.5-kDa trichloroacetic-acid-soluble translational inhibitor protein from human monocytes that is upregulated upon cellular differentiation [J].

Schmiedeknecht, G ;

Kerkhoff, C ;

Orso, E ;

Stohr, J ;

Aslanidis, C ;

Nagy, GM ;

Knuechel, R ;

Schmitz, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 242 (02) :339-351

← 1 2 →