Analytical strategies for characterization of bile acid and oxysterol metabolomes

被引：29

作者：

Griffiths, William J. ^{[2
]}

Sjovall, Jan ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophysics, SE-17177 Stockholm, Sweden

[2] Swansea Univ, Sch Med, Inst Mass Spectrometry, Swansea SA2 8PP, W Glam, Wales

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 396卷 / 01期

基金：

瑞典研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

Steroid; Mass spectrometry; Ion exchange; Derivatization; Lipidome; CHROMATOGRAPHY-MASS-SPECTROMETRY; LIVER X RECEPTORS; PROGESTERONE METABOLITES; URINE; PROFILES; IDENTIFICATION; STEROIDS; PLASMA; ELIMINATION; CHOLESTEROL;

D O I：

10.1016/j.bbrc.2010.02.149

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Cholesterol is the precursor of many compounds with functions in the physiology and metabolism of the organism. Methods for the multicomponent analysis of these compounds and their metabolites (metabolomics) are needed to improve our understanding of their roles in different species, organs, cells and metabolic situations and to clarify structure/activity relationships. This review discusses methods based on combinations of ion exchange and reversed-phase separations for sample preparation with derivatization and "charge-tagging" for chromatography-mass spectrometry in qualitative and quantitative characterizations of oxysterol, bile alcohol, bile acid, and steroid hormone metabolomes. Advantages, disadvantages and potential improvements for high-throughput applications are briefly discussed. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：80 / 84

页数：5

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