Replication-competent retroviral vectors for expressing genes in avian cells in vitro and in vivo

被引：18

作者：

Bell, EJD

Brickell, PM

机构：

[1] UMDS, GUYS HOSP, DEPT DEV NEUROBIOL, LONDON, ENGLAND

[2] INST CHILD HLTH, MOL HAEMATOL UNIT, LEUKAEMIA RES FUND CTR CHILDHOOD LEUKAEMIA, LONDON WC1N 1EH, ENGLAND

来源：

MOLECULAR BIOTECHNOLOGY | 1997年 / 7卷 / 03期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

retrovirus; vector; rous sarcoma virus; RCAS; chick embryo;

D O I：

10.1007/BF02740819

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Replication-competent retroviral vectors based on Rous sarcoma virus (RSV) are becoming increasingly popular for expressing genes in both primary cell cultures and embryonic chick tissues in ovo. In this article we review the features of RSV and its life cycle that make it suitable for use as a vector. We describe the design and use of the RCA and RCAS(BP) series of vectors which are currently the most widely used RSV-based vectors, illustrating both their strengths and weaknesses. Finally, we outline laboratory protocols suitable for the handling of these retroviral vectors.

引用

页码：289 / 298

页数：10

共 45 条

[1] ENDOGENOUS VIRAL GENES ARE NON-ESSENTIAL IN THE CHICKEN [J].