Background priming during reverse transcription by oligo(dT) carried over from mRNA isolation

被引：10

作者：

Piché, C ^{[1
]}

Schernthaner, JP ^{[1
]}

机构：

[1] Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada

来源：

BIOTECHNIQUES | 2003年 / 34卷 / 04期

关键词：

D O I：

10.2144/03344bm08

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：720 / +

页数：3

共 12 条

[1]

AASHEIM HC, 1994, BIOTECHNIQUES, V16, P716

[2]

Carninci P, 1997, DNA Res, V4, P61, DOI 10.1093/dnares/4.1.61

[3]

FRECH B, 2003, NUCLEIC ACIDS RES, V22, P4342

[4]

JACOBSEN KS, 1990, NUCLEIC ACIDS RES, V18, P3639

[5] Characterization of RNA strand displacement synthesis by moloney murine leukemia virus reverse transcriptase [J].

Kelleher, CD ;

Champoux, JJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (16) :9976-9986

[6] CDNA LIBRARY CONSTRUCTION FROM SMALL AMOUNTS OF RNA USING PARAMAGNETIC BEADS AND PCR [J].

LAMBERT, KN ;

WILLIAMSON, VM .

NUCLEIC ACIDS RESEARCH, 1993, 21 (03) :775-776

[7] REUSABLE CDNA LIBRARIES COUPLED TO MAGNETIC BEADS [J].

LEE, YH ;

VACQUIER, VD .

ANALYTICAL BIOCHEMISTRY, 1992, 206 (01) :206-207

[8] Oligo(dT) primer generates a high frequency of truncated cDNAs through internal poly(A) priming during reverse transcription [J].

Nam, DK ;

Lee, S ;

Zhou, GL ;

Cao, XH ;

Wang, C ;

Clark, T ;

Chen, JJ ;

Rowley, JD ;

Wang, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (09) :6152-6156

[9] IMPROVED EFFICIENCY FOR SINGLE-SIDED PCR BY CREATING A REUSABLE POOL OF 1ST-STRAND CDNA COUPLED TO A SOLID-PHASE [J].

RAINERI, I ;

MORONI, C ;

SENN, HP .

NUCLEIC ACIDS RESEARCH, 1991, 19 (14) :4010-4010

[10]

Sambrook J., 2012, MOL CLONING LAB MANU

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