New monoclonal antibody for the sensitive detection of hydroxy-s-triazines in water by enzyme-linked immunosorbent assay

被引：13

作者：

Bruun, L

Koch, C

Jakobsen, MH

Aamand, J

机构：

[1] Geol Survey Denmark & Greenland, Dept Geochem, DK-2400 Copenhagen NV, Denmark

[2] Statens Serum Inst, Dept Appl Immunol, DK-2300 Copenhagen S, Denmark

[3] Exiqon AS, DK-2950 Vedbaek, Denmark

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 423卷 / 02期

关键词：

monoclonal antibody; hydroxytriazine; covalent immobilisation; immunoassay; herbicide; pesticide;

D O I：

10.1016/S0003-2670(00)01094-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article presents a new and sensitive immunoassay for the triazine metabolites hydroxypropazine, hydroxyatrazine and hydroxysimazine. The assay is based on covalent immobilisation of antigen in combination with an enzyme-labelled anti-hydroxy-s-triazine monoclonal antibody. This system enabled the development of an assay with variation coefficients below 3% and a detection limit below 0.01 mug/l hydroxyatrazine and hydroxypropazine. Analysis of hydroxyatrazine-spiked water of three different types yielded an average recovery of 102% at 0.1 mug/l hydroxyatrazine. Relative to hydroxyatrazine, assay cross-reactivity was 148% towards hydroxypropazine and 67% towards hydroxysimazine. (C)2000 Elsevier Science B.V. All rights reserved.

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收藏

页码：205 / 213

页数：9

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