Improvements in DNA sequencing by capillary electrophoresis at elevated temperature using poly(ethylene oxide) as a sieving matrix

被引：25

作者：

Wei, W

Yeung, ES ^{[1
]}

机构：

[1] Iowa State Univ, Ames Lab, USDOE, Ames, IA 50011 USA

[2] Iowa State Univ, Dept Chem, Ames, IA 50011 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2000年 / 745卷 / 01期

关键词：

DNA sequencing; poly(ethylene oxide);

D O I：

10.1016/S0378-4347(00)00069-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA sequencing in poly(ethylene oxide) (PEO) matrix by capillary electrophoresis was demonstrated at high temperature. The optimal separation temperature is around 40 degrees C. The effects of polymer concentration and types of buffers on the separation performance were investigated. A new buffer system consisting of Tris-Taps-His-EDTA works well with PEO. High-speed separation and good resolution can be fulfilled by using a single-MW PEO polymer. It offers similar separation performance as before for the small DNA fragments, but better performance for large DNA fragments. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：221 / 230

页数：10

共 32 条

[1]

[Anonymous], 1976, POLYETHYLENE OXIDE

[2]

BASKIN J, 1996, APPL THEOR ELECTROPH, V6, P23

[3] UNIVERSAL PROPERTIES OF SEMIDILUTE POLYMER-SOLUTIONS - A COMPARISON BETWEEN EXPERIMENTS AND THEORY [J].