Characterization and antioxidant activities of the polysaccharides from mycelium of Phellinus pini and culture medium

被引:36
作者
Jiang, Peng [1 ]
Yuan, Lei [2 ]
Cai, Dongling [1 ]
Jiao, Lili [3 ]
Zhang, Liping [1 ]
机构
[1] NE Normal Univ, Sch Life Sci, Changchun 130024, Peoples R China
[2] Tibet Univ, Agr & Anim Husb Coll, Lhasa 860000, Xizang, Peoples R China
[3] Changchun Univ Chinese Med, Ginseng Acad, Changchun 130117, Peoples R China
关键词
Polysaccharides; Phellinus pini; Antioxidant activity; LINTEUS;
D O I
10.1016/j.carbpol.2014.10.013
中图分类号
O69 [应用化学];
学科分类号
070301 [无机化学];
摘要
Two polysaccharides isolated and purified from the mycelium (PPM) and its culture medium (PPE) of Phellinus pini using gel filtration were subjected to composition analysis and valuated for the antioxidant activity. The average molecular weights of PPM and PPE were approximately 22.0 and 38.0 kDa, respectively. PPM and PPE were both neutral heteropolysaccharides consisting of mannose, galactose, and glucose with molecular ratios of 2.99:1.00:0.34 and 38.40:1.00:1.76, respectively. In vitro antioxidant assay, PPM and PPE could scavenge 1,1-dipheny1-2-picrylhydrazyl (DPPH) radical and hydroxyl radical, chelate ferrous ion and reduce ferric ion. The antioxidant activities of PPM were stronger than those of PPE, suggesting that PPM has significant potential as a natural antioxidant agent. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:600 / 604
页数:5
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