A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I-V

被引:276
作者
Boye, K. [1 ]
Bartels, M. D. [1 ]
Andersen, I. S. [1 ]
Moller, J. A. [1 ]
Westh, H. [1 ]
机构
[1] Hvidovre Univ Hosp, Dept Clin Microbiol 445, DK-2650 Hvidovre, Denmark
关键词
identification; molecular; MRSA; multiplex PCR; SCCmec type; Staphylococcus aureus;
D O I
10.1111/j.1469-0691.2007.01720.x
中图分类号
R51 [传染病];
学科分类号
100401 [流行病与卫生统计学];
摘要
A multiplex PCR with four primer-pairs was designed to identify the five main known SCCmec types. A clear and easily discriminated band pattern was obtained for all five types. The SCCmec type was identified for 98% of 312 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). SCCmec type IV was by far the most common SCCmec type among both hospital- and community-acquired MRSA isolates in Denmark.
引用
收藏
页码:725 / 727
页数:3
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