ITIH3 Is a Potential Biomarker for Early Detection of Gastric Cancer

被引：66

作者：

Chong, Poh Kuan ^{[1
]}

Lee, Huiyin

Zhou, Jianbiao

Liu, Shaw-Cheng

Loh, Marie Chiew Shia

Wang, Ting Ting

Chan, Siew Pang ^{[2
]}

Smoot, Duane T. ^{[3
,4
]}

Ashktorab, Hassan ^{[3
,4
]}

So, Jimmy Bok Yan ^{[5
]}

Lim, Khong Hee ^{[6
]}

Yeoh, Khay Guan ^{[7
]}

Lim, Yoon Pin ^{[8
,9
]}

机构：

[1] Natl Univ Singapore, Canc Sci Inst Singapore, Ctr Life Sci, Singapore 117456, Singapore

[2] Singapore Inst Management Univ, Singapore 599491, Singapore

[3] Howard Univ, Dept Med, Washington, DC 20060 USA

[4] Howard Univ, Ctr Canc, Washington, DC 20060 USA

[5] Natl Univ Singapore Hosp, Dept Surg, Singapore 119074, Singapore

[6] Tan Tock Seng Hosp, Dept Surg, Singapore 308433, Singapore

[7] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Med, Singapore 65166914, Singapore

[8] Natl Univ Singapore, Fac Sci, Dept Biol Sci, Singapore 117546, Singapore

[9] Agcy Sci Technol & Res, Bioinformat Inst, Singapore 138668, Singapore

来源：

JOURNAL OF PROTEOME RESEARCH | 2010年 / 9卷 / 07期

基金：

英国医学研究理事会; 新加坡国家研究基金会;

关键词：

ITIH3; gastric cancer; plasma; proteomics; mouse xenograft; ALPHA-TRYPSIN INHIBITOR; BREAST-CANCER; PLASMA PROTEOME; PEPSINOGEN TEST; HEAVY-CHAIN; MOUSE MODEL; KINASE; IDENTIFICATION; EXPRESSION; CARCINOMA;

D O I：

10.1021/pr100192h

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Gastric cancer has one of the highest morbidities and mortalities worldwide. Early detection is key measure to improve the outcome of gastric cancer patients. In our efforts to identify potential markers for gastric cancer detection, we coupled xenotransplantation mouse model with a plasma proteomic approach. MKN45 gastric cancer cells were subcutaneously injected into nude mice and plasma samples from mice bearing different sizes of tumors were collected and subjected to iTRAQ and mass spectrometry analysis. ITIH3 protein was found to be more highly expressed in plasma of tumor bearing mice compared to control. Subsequent screening of ITIH3 expression in 167 clinical plasma samples, including 83 cancer-free subjects and 84 gastric cancer patients, revealed higher ITIH3 level in the plasma of gastric cancer patients. A receiver operating characteristics (ROC) curve estimated a maximal sensitivity of 96% at 66% specificity for ITIH3 in gastric cancer detection. In addition, plasma from early stage gastric cancer patient has significantly (p < 0.001) higher level of ITIH3 compared to that from noncancer subject. Our data suggest that ITIH3 may be a useful biomarker for early detection of gastric cancer.

引用

页码：3671 / 3679

页数：9

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