Surface plasmon resonance immunosensor for bacteria detection

被引：62

作者：

Baccar, H. ^{[1
,2
]}

Mejri, M. B. ^{[1
,3
]}

Hafaiedh, I. ^{[1
,2
]}

Ktari, T. ^{[1
]}

Aouni, M. ^{[3
]}

Abdelghani, A. ^{[1
,2
]}

机构：

[1] Ctr Urbain Nord, INSAT, Nanotechnol Lab, Charguia 1080, Tunisia

[2] IPEST, Unite Rech Physicochim Mat Polymeres, La Marsa 2070, Tunisia

[3] Fac Pharm, Lab Malad Transmissibles & Subst Biologiquement A, Monastir 5000, Tunisia

来源：

TALANTA | 2010年 / 82卷 / 02期

关键词：

SPR; Biofunctionalization; Immunosensor; Gold nanoparticles;

D O I：

10.1016/j.talanta.2010.05.060

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work describes an approach for the development of two bacteria biosensors based on surface plasmon resonance (SPR) technique. The first biosensor was based on functionalized gold substrate and the second one on immobilized gold nanoparticles. For the first biosensor, the gold substrate was functionalized with acid-thiol using the self-assembled monolayer technique, while the second one was functionalized with gold nanoparticles immobilized on modified gold substrate. A polyclonal anti-Escherichia coli antibody was immobilized for specific (E. coli) and non-specific (Lactobacillus) bacteria detection. Detection limit with a good reproducibility of 10(4) and 10(3) cfu mL(-1) of E. coli bacteria has been obtained for the first biosensor and for the second one respectively. A refractive index variation below 5 x 10(-3) due to bacteria adsorption is able to be detected. The refractive index of the multilayer structure and of the E. coli bacteria layer was estimated with a modeling software. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：810 / 814

页数：5

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