CAPS and DHPLC analysis of a single nucleotide polymorphism in the cytochrome b gene conferring resistance to strobilurins in field isolates of Blumeria graminis f. sp hordei

被引：22

作者：

Bäumler, S ^{[1
]}

Felsenstein, FG ^{[1
]}

Schwarz, G ^{[1
]}

机构：

[1] Tech Univ Munich, Chair Agron & Plant Breeding, Ctr Life & Food Sci Weihenstephan, Munich, Germany

来源：

JOURNAL OF PHYTOPATHOLOGY | 2003年 / 151卷 / 03期

关键词：

Blumeria graminis f. sp hordei; fungicide resistance; strobilurin; QoI fungicide; CAPS; DHPLC;

D O I：

10.1046/j.1439-0434.2003.00699.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A single nucleotide polymorphism in the wheat powdery mildew (Blumeria graminis f. sp. tritici) cytochrome b gene is responsible for resistance to inhibitors of the quinol outer binding site of the cytochrome bc(1) complex (QoI) fungicides. Analysis of a partial sequence of the cytochrome b gene from field isolates resistant and sensitive to QoI fungicides revealed the same point mutation in barley powdery mildew (B. graminis f. sp. hordei). Analysis of 118 and 40 barley powdery mildew isolates using a cleaved amplified polymorphic sequence assay and denaturing high performance liquid chromatography, respectively, confirmed that this single nucleotide polymorphism also confers resistance to QoI fungicides in barley powdery mildew.

引用

页码：149 / 152

页数：4

共 10 条

[1] STROBILURINS - NEW ANTIFUNGAL ANTIBIOTICS FROM BASIDIOMYCETE STROBILURUS-TENACELLUS (PERS EX FR) SING [J].