Epidemiology and molecular characterization of a clone of Burkholderia cenocepacia responsible for nosocomial pulmonary tract infections in a French intensive care unit

被引：20

作者：

Graindorge, Arnault ^{[1
,2
,3
]}

Menard, Aymeric ^{[1
,2
,3
]}

Neto, Manuelle ^{[1
,2
,3
]}

Bouvet, Claude ^{[4
]}

Miollan, Roger ^{[4
]}

Gaillard, Sandrine ^{[4
]}

de Montclos, Henri ^{[4
]}

Laurent, Frederic ^{[1
,2
,3
,4
]}

Cournoyer, Benoit ^{[1
,2
,3
]}

机构：

[1] Univ Lyon 1, CNRS, Ecol Microbienne UMR5557, Res Grp Bacterial Opportunist Pathogens & Environ, F-69622 Villeurbanne, France

[2] Univ Lyon, F-69361 Lyon, France

[3] Ecole Natl Vet Lyon, F-69280 Marcy Letoile, France

[4] Ctr Hosp Bourg En Bresse, F-01012 Bourg En Bresse, France

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2010年 / 66卷 / 01期

关键词：

Nosocomial outbreak; Burkholderia cenocepacia; B; cepacia; vietnamiensis; multivorans; Pneumonia; Cystic fibrosis; CYSTIC-FIBROSIS PATIENTS; CEPACIA COMPLEX; PSEUDOMONAS-CEPACIA; SP-NOV; OUTBREAK; IDENTIFICATION; SEQUENCE; STRAINS; GENE; BACTEREMIA;

D O I：

10.1016/j.diagmicrobio.2009.06.008

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Clustered cases of nosocomial pulmonary infections were observed in a French intensive care unit. Biochemical tests showed the etiologic agents to be part of the Bcc (Bcc). recA polymerase chain reaction-restriction fragment length polymorphism analysis and molecular phylogeny positioned the isolates into Burkholderia cenocepacia. Their recA sequences were found identical to those of ET12 strains responsible of necrotic pneumonia in cystic fibrosis patients. Analyses of a multi locus sequence typing genes set confirmed this proximity and suggested a wide distribution among occidental countries but could not resolve their phylogenetic position unambiguously. A novel marker, ecfB, indicated a significant phylogenetic divergence from ET12 strains. Pulse field gel electrophoresis analysis of SpeI-restricted total genomic DNA of the strains showed a unique profile indicative of a clonal outbreak. Environmental hospital screenings indicated cross-contamination between staff and patients. Bcc strains from outdoor environments were not related to this clone but indicated the presence of Burkholderia multivorans and Burkholderia vietnamiensis. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：29 / 40

页数：12

共 61 条

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