Differential effects of combined N sources on early steps of the nod factor-dependent transduction pathway in Lotus japonicus

被引:70
作者
Barbulova, Ani [1 ]
Rogato, Alessandra [1 ]
D'Apuzzo, Enrica [1 ]
Omrane, Selim [1 ]
Chiurazzi, Maurizio [1 ]
机构
[1] Inst Genet & Biophys A, I-80131 Naples, Italy
关键词
D O I
10.1094/MPMI-20-8-0994
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The development of nitrogen-fixing nodules in legumes is induced by perception of lipoehitin-oligosaccharide signals secreted by a bacterial symbiont. Nitrogen (N) starvation is a prerequisite for the formation, development, and function of root nodules, and high levels of combined N in the form of nitrate or ammonium can completely abolish nodule formation. We distinguished between nitrate and ammonium inhibitory effects by identifying when and where these combined N sources interfere with the Nod-factor-induced pathway. Furthermore, we present a small-scale analysis of the expression profile, under different N conditions, of recently identified genes involved in the Nod-factor-induced pathway. In the presence of high levels of nitrate or ammonium, the NIN gene fails to be induced 24 h after the addition of Nod factor compared with plants grown under N-free conditions. This induction is restored in the hypernodulating nitrate-tolerant har1-3 mutant only in the presence of 10 and 20 mM KNO3. These results were confirmed in Lotus plants inoculated with Mesorhizobium loti. NIN plays a key role in the nodule organogenesis program and its downregulation may represent a crucial event in the nitrate-dependent pathway leading to the inhibition of nodule organogenesis.
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页码:994 / 1003
页数:10
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