In vivo fluorescent imaging of the mouse retina using adaptive optics

被引：53

作者：

Biss, David P.

Sumorok, Daniel

Burns, Stephen A.

Webb, Robert H.

Zhou, Yaopeng

Bifano, Thomas G.

Cote, Daniel

Veilleux, Israel

Zamiri, Parisa

Lin, Charles P.

机构：

[1] Schepens Eye Res Inst, Boston, MA 02114 USA

[2] Boston Univ, Brookline, MA 02446 USA

[3] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Wellman Ctr Photomed,Adv Microscopy Program, Boston, MA 02114 USA

来源：

OPTICS LETTERS | 2007年 / 32卷 / 06期

关键词：

D O I：

10.1364/OL.32.000659

中图分类号：

O43 [光学];

学科分类号：

070207 [光学]; 0803 [光学工程];

摘要：

In vivo imaging of the mouse retina using visible and near infrared wavelengths does not achieve diffraction-limited resolution due to wavefront aberrations induced by the eye. Considering the pupil size and axial dimension of the eye, it is expected that unaberrated imaging of the retina would have a transverse resolution of 2 mu m. Higher-order aberrations in retinal imaging of human can be compensated for by using adaptive optics. We demonstrate an adaptive optics system for in vivo imaging of fluorescent structures in the retina of a mouse, using a microelectromechanical system membrane mirror and a Shack-Hartmann wavefront sensor that detects fluorescent wavefront. (c) 2007 Optical Society of America.

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收藏

页码：659 / 661

页数：3

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