RNA:protein interactions associated with satellites of panicum mosaic virus

被引：22

作者：

Desvoyes, B ^{[1
]}

Scholthof, KBG ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77845 USA

来源：

FEBS LETTERS | 2000年 / 485卷 / 01期

关键词：

panicum mosaic virus; satellite virus; satellite RNA; encapsidation;

D O I：

10.1016/S0014-5793(00)02177-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interactions between satellite panicum mosaic virus (SPMV) capsid protein (CP) and its 824 nucleotide (nt) single stranded RNA were investigated by gel mobility shift assay and Northwestern blot assay. SPMV CP has specificity for its RNA at high affinity, but little affinity for non-viral RNA. The SPMV CP also bound a 350 nt satellite RNA (satRNA) that, like SPMV, is dependent on panicum mosaic virus for its replication. SPMV CP has the novel property of encapsidating SPMV RNA and satRNA. Competition gel mobility shift assays performed with a non-viral RNA and unlabeled SPMV RNA and satRNA revealed that these RNA:protein interactions mere in part sequence specific. (C) 2000 Federation of European Biochemical Societies. Published by EIsevier Science B.V. All rights reserved.

引用

页码：25 / 28

页数：4

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