A new flavonol, brevicornin, with a 3''-methoxy-isopentyl group, was isolated from the aerial parts of Epimedium brevicornum. Its structure was determined to be 3,5,7-trihydroxy-4'-methoxy-8-(3''-methoxyisopentyl) flavone. Four known flavonoids epimedin B, epimedin C, beta-anhydroicaritin and tricin were also isolated from the same plant.