A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction

被引：76

作者：

Kaminishi, Tatsuya

Wilson, Daniel N.

Takemoto, Chie

Harms, Joerg M.

Kawazoe, Masahito

Schluenzen, Frank

Hanawa-Suetsugu, Kyoko

Shirouzu, Mikako

Fucini, Paola

Yokoyama, Shigeyuki

机构：

[1] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

[2] RIKEN, Genom Sci Ctr, Yokohama, Kanagawa 2300045, Japan

[3] Univ Tokyo, Grad Sch Sci, Dept Biochem & Biophys, Bunkyo Ku, Tokyo 1130033, Japan

来源：

STRUCTURE | 2007年 / 15卷 / 03期

关键词：

D O I：

10.1016/j.str.2006.12.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the initiation phase of bacterial translation, the 30S ribosomal subunit captures mRNA in preparation for binding with initiator tRNA. The purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of the mRNA, anchors the 30S subunit near the start codon, via base pairing with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we present the 3.3 angstrom crystal structure of the Thermus thermophilus 30S subunit bound with an mRNA mimic. The duplex formed by the SD and aSD sequences is snugly docked in a "chamber" between the head and platform domains, demonstrating how the 30S subunit captures and stabilizes the otherwise labile SD helix. This location of the SD helix is suitable for the placement of the start codon AUG in the immediate vicinity of the mRNA channel, in agreement with reported crosslinks between the second position of the start codon and G1530 of 16S rRNA.

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页码：289 / 297

页数：9

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