Competitive displacement of β-lactoglobulin from the air/water interface by sodium dodecyl sulfate

被引:116
作者
Mackie, AR [1 ]
Gunning, AP [1 ]
Wilde, PJ [1 ]
Morris, VJ [1 ]
机构
[1] Inst Food Res, Dept Food Qual & Mat Sci, Norwich NR4 7UA, Norfolk, England
关键词
D O I
10.1021/la0003950
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In this work, the displacement of a globular protein from the air/water interface by sodium dodecyl sulfate (SDS) was fallowed using a combination of the Langmuir-Blodgett method and atomic farce microscopy. The results are compared to displacement of the same protein by the nonionic surfactant Tween 20. The displacement by SDS is characterized by the same orogenic mechanism but the charge inter actions between the protein and surfactant settled to increase the surface pressure at which displacement occurred. Two types of interfacial film transfer were used in order to check that features seen were not caused by the method of transfer. The displacement was also followed using two surface rheological techniques: shear and dilation. The results from the rheological studies are correlated with the data seen with the AFM and show that the mixed film was much weaker than either of the component films. Both surface sheaf and dilation rheology showed similar trends. Also demonstrated is the appearance of larger circular holes in the protein film during the latter stages of displacement.
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收藏
页码:8176 / 8181
页数:6
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