Reductive dehalogenation of chlorobenzene congeners in cell extracts of Dehalococcoides sp strain CBDB1

被引:70
作者
Hölscher, T [1 ]
Görisch, H [1 ]
Adrian, L [1 ]
机构
[1] Tech Univ Berlin, FG Tech Biochem, Inst Biotechnol, D-13353 Berlin, Germany
关键词
D O I
10.1128/AEM.69.5.2999-3001.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Enzymatic reductive dehalogenation of tri-, tetra-, penta-, and hexachlorobenzenes was demonstrated in cell extracts with low protein concentration (0.5 to 1 mug of protein/ml) derived from the chlorobenzene-respiring anaerobe Dehalococcoides sp. strain CBDB1. 1,2,3-trichlorobenzene dehalogenase activity was associated with the membrane fraction. Light-reversible inhibition by alkyl iodides indicated the presence of a corrinoid cofactor.
引用
收藏
页码:2999 / 3001
页数:3
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