Fluorescent measurement of microalgal neutral lipids

被引：203

作者：

Elsey, Danielle

Jameson, David

Raleigh, Barry

Cooney, Michael J.

机构：

[1] Univ Hawaii Manoa, Sch Ocean & Earth Sci & Technol, Hawaii Nat Energy Inst, Honolulu, HI 96822 USA

[2] Univ Hawaii, John A Burns Sch Med, Dept Cell & Mol Biol, Honolulu, HI 96813 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 68卷 / 03期

关键词：

Nile Red (9-diethylamino-5H-benzo[alpha]phenoxazine-5-one); fluorescent probe; neutral lipids; microalgae; high-throughput screen;

D O I：

10.1016/j.mimet.2006.11.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nile Red, a dye that fluoresces at defined wavelengths depending upon the polarity of the surrounding medium, has been proposed to determine the neutral lipid content of microalgal cells. Herein we communicate modifications to this technique that facilitate its use as a high-throughput screening technology, as well as improving its accuracy and versatility. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：639 / 642

页数：4

共 19 条

[1] Automated GC-MS analysis of raw biological samples - Application to fatty acid profiling of aquatic micro-organisms [J].