Determination of quercetin in human plasma after ingestion of commercial canned green tea by semi-micro HPLC with electrochemical detection

被引：44

作者：

Jin, D

Hakamata, H

Takahashi, K

Kotani, A

Kusu, F

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Analyt Chem, Hachioji, Tokyo 1920392, Japan

[2] Yanbian Univ, Dept Chem Sci, Yanji 133002, Peoples R China

[3] Yanbian Univ, Coll Engn, Yanji 133002, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2004年 / 18卷 / 09期

关键词：

quercetin; human plasma; green tea; electrochemical detection; microbore column;

D O I：

10.1002/bmc.370

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Determination of quercetin in human plasma was carried out by semi-micro high-performance liquid chromatography with electrochemical detection. Peak heights for quercetin were found to be linearly related to the amount of each quercetin injected from 1.5 to 750 pg. The detection limit (signal-noise ratio, S/N = 3) of the present method for quercetin was 0.3 pg. Glucuronic and sulfate forms of quercetin in plasma were hydrolyzed enzymatically using beta-glucuronidase and sulfatase, respectively. Quercetin in plasma and the hydrolyzed solution were extracted with ethyl acetate and determined by the present method. The time courses of concentrations of quercetin in human plasma showed maxima at 1-1.5 h after ingestion of 340 mL of commercial canned green tea. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：662 / 666

页数：5

共 23 条

[1]

SELECTIVE EXTRACTION OF QUERCETRIN IN VEGETABLE DRUGS AND URINE BY OFF-LINE COUPLING OF BORONIC ACID AFFINITY-CHROMATOGRAPHY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].