A method for the rapid analysis of neuronal proportions and neurite morphology in primary cultures

被引：10

作者：

Isaacs, KR

Hanbauer, I

Jacobwitz, DM

机构：

[1] NIMH, Sect Histopathol, Clin Sci Lab, Bethesda, MD 20892 USA

[2] NHLBI, Lab Mol Immunol, Bethesda, MD 20892 USA

来源：

EXPERIMENTAL NEUROLOGY | 1998年 / 149卷 / 02期

关键词：

tyramide; fluorescence immunohistochemistry; tissue culture; Sholl analysis;

D O I：

10.1006/exnr.1997.6727

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

This article provides basic guidelines for a rapid analysis of subpopulation proportions and neurite morphology in primary cultures. We describe, in E14 mesencephalic primary cultures, an immunohistochemical method for the simultaneous identification of multiple neuronal phenotypes and an estimation of the ratio of subpopulations. In addition, we describe the use of the Renaissance TSA-Direct hit (NEN, DuPont) to enhance the visualization of neurites when the antigen is in low abundance. Finally, a modified SholI analysis is used to rapidly and reliably estimate neurite number and length.

引用

收藏

页码：464 / 467

页数：4

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