Enzymatic reduction of ketones in "micro-aqueous" media catalyzed by ADH-A from Rhodococcus ruber

被引：86

作者：

de Gonzalo, Gonzalo

Lavandera, Ivan

Faber, Kurt

Kroutil, Wolfgang

机构：

[1] Graz Univ, Dept Chem Organ & Bioorgan Chem, A-8010 Graz, Austria

[2] Graz Univ, Res Ctr Appl Biocatalysis, A-8010 Graz, Austria

来源：

ORGANIC LETTERS | 2007年 / 9卷 / 11期

关键词：

D O I：

10.1021/ol070679c

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Mono- and biphasic aqueous-organic solvent systems (50% v v(-1)) as well as micro-aqueous organic systems (99% v v(-1)) were successfully employed for the biocatalytic reduction of ketones catalyzed by alcohol dehydrogenase ADH-A from Rhodococcus ruber via hydrogen transfer. A clear correlation between the log P of the organic solvent and the enzyme activity-the higher, the better-was found. The use of organic solvents allowed highly stereoselective enzymatic carbonyl reductions at substrate concentrations close to 2.0 M.

引用

页码：2163 / 2166

页数：4

共 36 条

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