Interaction of tsg101 with Marburg virus VP40 depends on the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and tsg101 plays a critical role in the budding of Marburg virus-like particles induced by VP40, NP, and GP

被引：88

作者：

Urata, Shuzo

Noda, Takeshi

Kawaoka, Yoshihiro

Morikawa, Shigeru

Yokosawa, Hideyoshi

Yasuda, Jiro ^{[1
]}

机构：

[1] Natl Res Inst Police Sci, Dept Forens Sci 1, Kashiwa, Chiba 2770882, Japan

[2] Japan Sci & Technol Agcy, CREST, Saitama 3320012, Japan

[3] Hokkaido Univ, Grad Sch Pharmaceut Sci, Dept Biochem, Sapporo, Hokkaido 0600812, Japan

[4] Univ Tokyo, Inst Med Sci, Int Res Ctr Infect Dis, Tokyo 1088639, Japan

[5] Univ Wisconsin, Sch Vet Med, Dept Pathol Sci, Madison, WI 53706 USA

[6] Natl Inst Infect Dis, Dept Virol 1, Tokyo 2080011, Japan

来源：

JOURNAL OF VIROLOGY | 2007年 / 81卷 / 09期

关键词：

D O I：

10.1128/JVI.02829-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Marburg virus (MARV) VP40 is a matrix protein that can be released from mammalian cells in the form of virus-like particles (VLPs) and contains the PPPY sequence, which is an L-domain motif. Here, we demonstrate that the PPPY motif is important for VP40-induced VLP budding and that VLP production is significantly enhanced by coexpression of NP and GP. We show that Tsg101 interacts with VP40 depending on the presence of the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and plays an important role in VLP budding. These findings provide new insights into the mechanism of MARV budding.

引用

收藏

页码：4895 / 4899

页数：5

相关论文

共 39 条

[1] VP24 of Marburg virus influences formation of infectious particles [J].

Bamberg, S ;

Kolesnikova, L ;

Möller, P ;

Klenk, HD ;

Becker, S .

JOURNAL OF VIROLOGY, 2005, 79 (21) :13421-13433

[2] THE ASIALOGLYCOPROTEIN RECEPTOR IS A POTENTIAL LIVER-SPECIFIC RECEPTOR FOR MARBURG VIRUS [J].

BECKER, S ;

SPIESS, M ;

KLENK, HD .

JOURNAL OF GENERAL VIROLOGY, 1995, 76 :393-399

[3] PPPYEPTAP motif is the late domain of human T-Cell leukemia virus type 1 GaG and mediates its functional interaction with cellular proteins Nedd4 and Tsg101 [J].

Bouamr, F ;

Melillo, JA ;

Wang, MQ ;

Nagashima, K ;

Los Santos, MD ;

Rein, A ;

Goff, SP .

JOURNAL OF VIROLOGY, 2003, 77 (22) :11882-11895

[4] Influenza a viruses with mutations in the M1 helix six domain display a wide variety of morphological phenotypes [J].

Burleigh, LM ;

Calder, LJ ;

Skehel, JJ ;

Steinhauer, DA .

JOURNAL OF VIROLOGY, 2005, 79 (02) :1262-1270

[5] Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding [J].

Garrus, JE ;

von Schwedler, UK ;

Pornillos, OW ;

Morham, SG ;

Zavitz, KH ;

Wang, HE ;

Wettstein, DA ;

Stray, KM ;

Côté, M ;

Rich, RL ;

Myszka, DG ;

Sundquist, WI .

CELL, 2001, 107 (01) :55-65

[6] EFFECT OF MUTATIONS AFFECTING THE P6 GAG PROTEIN ON HUMAN-IMMUNODEFICIENCY-VIRUS PARTICLE RELEASE [J].

GOTTLINGER, HG ;

DORFMAN, T ;

SODROSKI, JG ;

HASELTINE, WA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (08) :3195-3199

[7] A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: Implications for filovirus budding [J].

Harty, RN ;

Brown, ME ;

Wang, GL ;

Huibregtse, J ;

Hayes, FP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13871-13876

[8] A proline-rich motif within the matrix protein of vesicular stomatitis virus and rabies virus interacts with WW domains of cellular proteins: Implications for viral budding [J].

Harty, RN ;

Paragas, J ;

Sudol, M ;

Palese, P .

JOURNAL OF VIROLOGY, 1999, 73 (04) :2921-2929

[9] P6(GAG) IS REQUIRED FOR PARTICLE-PRODUCTION FROM FULL-LENGTH HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 MOLECULAR CLONES EXPRESSING PROTEASE [J].

HUANG, MJ ;

ORENSTEIN, JM ;

MARTIN, MA ;

FREED, EO .

JOURNAL OF VIROLOGY, 1995, 69 (11) :6810-6818

[10] RETRACTED: YRKL sequence of influenza virus M1 functions as the L domain motif and interacts with VPS28 and Cdc42 (Retracted article. See vol 80 pg 10289 2006) [J].

Hui, EKW ;

Barman, S ;

Tang, DHP ;

France, B ;

Nayak, DP .

JOURNAL OF VIROLOGY, 2006, 80 (05) :2291-2308

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