C-terminal end of v-src protein interacts with peptide coded by gadd7/adapt15-like RNA in two-hybrid system

The significant differences in the metastatic properties of hamster fibroblasts transformed by the Rous sarcoma virus (RSV) ere associated with mutations in the v-src carboxyterminal region, To identify the capacity of this region for protein-protein interaction the two-hybrid system was used. The cDNA clone (vseap1), producing the protein specifically bound with the v-src C-terminal part in yeast cells in vivo and in GST-fusion system in vitro was isolated, Vseap1 shared 68% of homology with stressful agents induced RNA-gadd7/adapt15. Two vseap1 specific messenger RNAs were identified: 0.9-kbp RNA expressed in all transformed cells and three times less in embryo fibroblasts; 3.1-kbp transcript was deleted in the cells with suppressed v-src activity and H2O2 resistance. (C) 1998 Federation of European Biochemical Societies.